| Project/Area Number |
15K14442
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservation of biological resources
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| Research Institution | National Museum of Nature and Science, Tokyo |
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Principal Investigator |
Yukawa Tomohisa 独立行政法人国立科学博物館, 植物研究部, グループ長 (50280524)
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| Co-Investigator(Kenkyū-buntansha) |
山田 明義 信州大学, 学術研究院農学系, 准教授 (10324237)
辻田 有紀 佐賀大学, 農学部, 准教授 (80522523)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUTSUMI Chie 独立行政法人国立科学博物館, 植物研究部, 研究主幹 (30455422)
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| Research Collaborator |
YAGAME Takahiro
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 菌根 / 保全 / 植物 / 共生 / 菌類 |
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| Outline of Final Research Achievements |
We developed new methods to resolve three central issues concerning efficient ex situ conservation of mycoheterotrophic plants, i. e., 1) isolation of particular fungi in association with the host plant species, 2) culture and preservation of isolated fungi, and 3) establishment of plant-fungi symbiosis. Regarding the first issue, mass isolation of target fungal species was accomplished by repeated washes of hyphae in aqueous solution of antibiotics. In relation to the second issue, covering of hyphae with humus from the habitat of target species facilitated the growth and subsequent formation of fruit bodies. On the last issue, mycoheterotrophic plant-fungi symbiosis was established in this study, while further experiments are needed to establish tripartite symbiosis involving an autotrophic plant, a fungus, and a mycoheterotrophic plant.
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