| Project/Area Number |
15K14448
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
Yonehara Shin 京都大学, 生命科学研究科, 教授 (00124503)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 細胞死 / 細胞増殖 / ヒストンH1 / エピジェネティクス |
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| Outline of Final Research Achievements |
FLASH/casp8ap2 is involved in cleavage and maturation of canonical histone mRNA. When the expression of FLASH is suppressed, progression of the cell cycle arrests in the S phase and cell death is induced. At this time, expression of canonical histone H1.4 was shown to be inhibited and expression of non-canonical histone H1t was induced. On the other hand, down-regulated expression of H1.4 in FLASH-expressing cells did not affect cell cycle progression, while cellular proliferation was suppressed by transient expression of exogenous H1t. We consider that the inhibition of cell cycle progression at the S phase by the downregulated expression of FLASH may be due to the expression of H1t, and the new function of H1t on cell cycle progression will be further analyzed by generation and analysis of H1t knockout cells.
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