| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
For faithful duplication of genetic information, DNA replication initiates from many distinct sites on eukaryotic chromosomes in a manner to be regulated by a spatiotemporal program, although the molecular mechanisms of regulation has not been uncovered. We discovered that the higher-order chromatin communication of the telomeres and internal late replication origin plays important role in replication-timing control. Using LacI-GFP fluorescent protein bound to lacO repeat inserted nearby replication origins in fission yeast Shizosaccharomyces pombe, we found that late replication origins are localized at the nuclear periphery and adjacent to telomeres especially in G1/S phase when replication-timing is determined. Telomere-binding protein complex “shelterin” is required for telomere-association of the internal replication origins, although the nuclear peripheral localization is not important. These finding provide insight into a novel mechanism of replication control by telomeres.
|
