| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this research, we tried to establish a new approach to obtain the candidate proteins that control the target specificity of epigenetic modification enzymes by utilizing CRISPR/dCas9-based enChIP (engineered DNA-binding molecule-mediated chromatin immunoprecipitation). We found that dCas9 can bind to the target promoter region with high-specificity and without affecting DNA methylation status. However, binding of dCas9 significantly decreased the target gene expression, especially when binding region of dCas9 is located adjacent to the transcription start site. Our findings suggest that CRISPR/dCas9-based enChIP has limited utility in the adaptable regions but could be applied as an approach in other genomic regions.
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