A novel approach to the plasticity of region dependent regulation of DNA methylation
Project/Area Number |
15K14452
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | Kyushu University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 神経科学 / エピジェネティクス / DNAメチル化 |
Outline of Final Research Achievements |
In this research, we tried to establish a new approach to obtain the candidate proteins that control the target specificity of epigenetic modification enzymes by utilizing CRISPR/dCas9-based enChIP (engineered DNA-binding molecule-mediated chromatin immunoprecipitation). We found that dCas9 can bind to the target promoter region with high-specificity and without affecting DNA methylation status. However, binding of dCas9 significantly decreased the target gene expression, especially when binding region of dCas9 is located adjacent to the transcription start site. Our findings suggest that CRISPR/dCas9-based enChIP has limited utility in the adaptable regions but could be applied as an approach in other genomic regions.
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Report
(3 results)
Research Products
(5 results)
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[Journal Article] Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions.2016
Author(s)
Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I
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Journal Title
Nat Biotechnol.
Volume: 34
Issue: 10
Pages: 1060-1065
DOI
Related Report
Peer Reviewed
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[Presentation] ゲノム編集を利用したエピゲノムの書き換え2016
Author(s)
森田純代, 野口浩史, 堀居拓郎, 中林一彦, 木村美香, 岡村浩司, 坂井 淳彦, 中嶋 秀行,秦健一郎, 中島欽一, ○畑田出穂
Organizer
第39回日本分子生物学会年会
Place of Presentation
パシフィコ横浜
Year and Date
2016-11-30
Related Report
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[Presentation] CRISPR/Casを用いたエピゲノム編集法2016
Author(s)
森田純代, 野口浩史, 堀居拓郎, 中林一彦, 木村美香, 岡村浩司, 坂井 淳彦, 中嶋 秀行,秦健一郎, 中島欽一, ○畑田出穂
Organizer
日本核酸医薬学会 第2回年会
Place of Presentation
東京理科大学
Year and Date
2016-11-15
Related Report
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