| Project/Area Number |
15K14453
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Keio University |
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Principal Investigator |
Ko Minoru 慶應義塾大学, 医学部(信濃町), 教授 (50631199)
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| Co-Investigator(Kenkyū-buntansha) |
石黒 啓一郎 熊本大学, 発生医学研究所, 准教授 (30508114)
洪 繁 慶應義塾大学, 医学部(信濃町), 准教授 (90402578)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Zscan4 / Zygotic genss / GV oocytes / 減数分裂関連因子 / ES細胞 / 減数分裂 |
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| Outline of Final Research Achievements |
Zscan4 is known to be expressed in the 2 cell stage mouse embryos and is characterized by activation of the zygotic genes and ectopic expression of meiosis-related genes. In this study, ES cells and mice in which GFP was knocked-in at the endogenous Zscan4c locus were prepared, and the expression pattern of endogenous Zscan4 was analyzed. As a result, it was found that the endogenous Zscan4c in ES cells is about 30% of all Zscan4 positive cells. Expression of Zscan4 was observed in GV oocytes corresponding to late stage of meiotic prophase and spermatocytes at Pachytene stage.
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