| Project/Area Number |
15K14454
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
Jozuka Katsuki 基礎生物学研究所, 多様性生物学研究室, 助教 (40291893)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 染色体 / クロマチン / 細胞分裂 / 分子生物学 / 遺伝学 / 染色体構造 / ヘテロクロマチン / FISH / コンデンシン / rolling circle |
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| Outline of Final Research Achievements |
In this study, I showed that the condensin complex, a key player for the mitotic chromosome condensation, controls the chromosome folding even in interphase cells. First, I found the difference in the chromosome 3 folding state between two mating types, a- and alpha-type cells. Condensin controls a-type cell specific chromosome folding. I found the a-type cell specific condensin binding element on chromosome 3. This cis-element is required for the a-type cell specific folding of chromosome 3 and works even at ectopic positions on chromosome 3. Furthermore, it was found that both condensin and the cis-element control a frequency of mating-type switching into opposite type. These results suggest that condensin regulate a mating-type conversion by controlling chromosome 3 folding state.
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