| Project/Area Number |
15K14456
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KATOH Yohei 京都大学, 大学院薬学研究科, 助教 (90568172)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | タンパク質間相互作用 / GFP / Nanobody / 繊毛内タンパク質輸送複合体 / マイクロプレート / ポリスチレン親和性タグ |
|---|
| Outline of Final Research Achievements |
The visible immunoprecipitation (VIP) assay is a convenient, flexible method to detect protein-protein interactions. In this study, I attempted to improve the VIP assay to detect protein-protein interactions on a microplate and enable high-throughput screening.
By fusing polystyrene (PS)-affinity peptide sequence to anti-GFP-Nanoboy, I successfully immobilized it to PS microplate. The sensitivity was further enhanced by ELISA, through optimizing the concentrations of the secondary and tertiary antibodies.
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