Analysis of signal molecule modifications by multidimensional flow cytometric method

• Project/Area Number: 15K14459
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kobe University
• Principal Investigator: Kawamoto Satoshi 神戸大学, バイオシグナル総合研究センター, 教室系技術職員 (60457116)
• Co-Investigator(Kenkyū-buntansha): 岩崎 哲史 神戸大学, バイオシグナル総合研究センター, 助教 (40379483)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: フローサイトメトリー / 細胞内シグナル伝達

Outline of Final Research Achievements

We tried to establish "Multidimensional flow cytometric analysis method" which could analyze multi parameters at the same time on a cell-by-cell basis by using flow cytometer and verify that it could be a tool for analyzing behavior of signaling molecules. By using the system, we analyzed gene expression and expression of phosphorylated protein in melanoma cell line at single cell level at the same time by examining various conditions of sample preparation. We also created a three-dimensional graph that simultaneously displays three or more parameters.

Research Products

• [Presentation] 多次元フローサイトメトリー解析法の確立 (2018)
  • Author(s): 川本 智
  • Organizer: 第29回生物学技術研究会