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Studies on small motion of endomembranes affecting reactions in the narrow space

Research Project

Project/Area Number 15K14462
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionFukushima Medical University

Principal Investigator

WADA IIKUO  福島県立医科大学, 医学部, 教授 (40182969)

Research Collaborator Yoshida Hiroshi  
Arai Seisuke  
Inoue Naokazu  
Hashimoto Hitoshi  
Takeuchi Mayumi  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords小胞体 / 揺らぎ / 細胞骨格 / 拡散 / 微小管 / 品質管理
Outline of Final Research Achievements

As reactions confined in the endomembrane system of eukaryotes occur in a small volume, the volume change may affect the kinetics. When endomembrane motion was observed at high frame rates, we noted small nonhomogeneous fluctuation of the endoplasmic reticulum (ER). Quantitative analyses using the trapped aggregates in the ER lumen revealed that this motion was unique to the ER and that the rate was nearly consistent in all cells examined. This motion was solely caused by the activity of the ER membranes and demonstrated no temperature dependence. By forcefully repressing by microtubules, we found that cargo motion was facilitated by the structure fluctuation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] Endoplasmic reticulum proteins SDF2 and SDF2L1 act as components of the BiP chaperone cycle to prevent protein aggregation.2017

    • Author(s)
      Fujimori T, Suno R, Iemura SI, Natsume T, Wada I, Hosokawa N
    • Journal Title

      Genes to cells

      Volume: 8 Issue: 8 Pages: 684-698

    • DOI

      10.1111/gtc.12506

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carboxyl-terminal tail-mediated homodimerizations of sphingomyelin synthases are responsible for efficient export from the endoplasmic reticulum2017

    • Author(s)
      Hayashi Y, Nemoto-Sasaki Y, Matsumoto N, Tanikawa T, Oka S, Tanaka Y, Arai S, Wada I, Sugiura T, Yamashita A.
    • Journal Title

      J Biol Chem.

      Volume: 292 Issue: 3 Pages: 1122

    • DOI

      10.1074/jbc.m116.746602

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Association of the SEL1L protein transmembrane domain with HRD1 ubiquitin ligase regulates ERAD-L2016

    • Author(s)
      Hoshikawa N, Wada I
    • Journal Title

      FEBS Journal

      Volume: 283 Issue: 1 Pages: 157-172

    • DOI

      10.1111/febs.13564

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oocyte-triggered dimerization of sperm IZUMO1 promotes sperm-egg fusion in mice2015

    • Author(s)
      Inoue K, Hagihara Y, Wright D, Suzuki T, Wada I
    • Journal Title

      Nature Communications

      Volume: 6 Issue: 1 Pages: 8858-8858

    • DOI

      10.1038/ncomms9858

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of Russell bodies accumulating the mutant antithrombin that derived from endoplasmic reticulum2015

    • Author(s)
      Kimura K, Kawaguchi K, Ueda Y, Arai S, Morita M, Imanaka T, Wada, I
    • Journal Title

      Biol. Pharm. Bull

      Volume: 38 Issue: 6 Pages: 852-861

    • DOI

      10.1248/bpb.b14-00773

    • NAID

      130005072668

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Endoplasmic Reticulum Stress Response and Mutant Protein Degradation in CHO Cells Accumulating Antithrombin (C95R) in Russell Bodies2015

    • Author(s)
      Kimura K, Inoue K, Okubo J, Ueda Y, Kawaguchi K, Sakurai H, Wada I, Morita M, Imanaka T.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 38 Issue: 12 Pages: 1980-1984

    • DOI

      10.1248/bpb.b15-00618

    • NAID

      130005112646

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 小胞体膜の微小揺動に関する研究2017

    • Author(s)
      荒井斉祐、橋本仁志、和田郁夫
    • Organizer
      第69回日本細胞生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Improvement of fluorescence proteins suitable for live-cell imaging in the oxidative environment in plant cells2017

    • Author(s)
      西村 浩二, 久我 一弘, 岩瀬 駿志, 和田 郁夫, 清水 英寿, 地阪 光生, 横田 一成, 中川 強
    • Organizer
      第59回日本植物生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] SEL1Lタンパク質による小胞体関連分解の調節機構2017

    • Author(s)
      細川暢子、和田郁夫
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 高精度イメージングが教えること2017

    • Author(s)
      和田郁夫
    • Organizer
      第22回日本臨床エンブリオロジスト学会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 小胞体の微小な揺らぎは細胞質反応を促進する2016

    • Author(s)
      荒井斉祐 , 橋本 仁志 , 井上 直和 , 和田郁夫
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2016 Research-status Report
  • [Presentation] 分泌系タンパク質イメージングのための蛍光タグの検討2015

    • Author(s)
      鈴木 貴久、和田郁夫
    • Organizer
      第38回 日本分子生物学会年会&第88回 日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 小胞体膜に存在するHRD1-SEL1Lユビキチンリガーゼ複合体の機能解析2015

    • Author(s)
      細川 暢子、和田 郁夫
    • Organizer
      第38回 日本分子生物学会年会&第88回 日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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