Biological significance of pseudouridylation in mRNAs
| Project/Area Number |
15K14466
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Research Collaborator |
IWAKI Megumi
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 転写後修飾 / シュードウリジン / mRNAリコーディング / 翻訳 / 遺伝情報再コード化 / mRNA |
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| Outline of Final Research Achievements |
To estimate the biological significance of uridine-to-pseudouridine (U-to-Psi) modification found in natural mRNAs, possible amino acid substitution was investigated in the protein produced from a Psi-containing mRNA for amyloid precursor protein (APP). A Psi was introduced at the second site of codon 435 in the APP mRNA partial fragment, and it was used as template for in vitro translation using wheat germ extract. As the resultant protein product was analyzed, its molecular mass was quite identical to that observed in the case that a relevant mRNA fragment without Psi modification was used as template. This result indicates that the amino acid code remains unchanged even when Psi is introduced in the codon UUC for phenylalanine, at least in the case of codon 435 in APP mRNA.
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Report
(3 results)
Research Products
(7 results)
