Structural elucidation of dioxin recognition by aryl hydrocarbon receptor
Project/Area Number |
15K14471
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
Ohto Umeharu 東京大学, 大学院薬学系研究科(薬学部), 准教授 (90451856)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | ダイオキシン / 環境ホルモン / 構造生物学 / X線結晶構造解析 / X線結晶解析 |
Outline of Final Research Achievements |
We conducted the structural biology of aryl hydrocarbon receptor (AhR), known as dioxin receptor. We tried to prepare the pre-nuclear translocation complex of AhR but it was not successful. We conducted the crystallization of the AhR inhibitory complex, AhRR/ARNT complex, and determined its crystal structure. To obtain the structural information of the AhR transcriptional activation complex, AhR/ARNT complex, we tried to prepare AhR/ARNT complex derived from various organism species, and expressed and purified various mutants of AhR/ARNT.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Structural Analysis Reveals that Toll-like Receptor 7 Is a Dual Receptor for Guanosine and Single-Stranded RNA2016
Author(s)
Zhang Z, Ohto U, Shibata T, Krayukhina E, Taoka M, Yamauchi Y, Tanji H, Isobe T, Uchiyama S, Miyake K, Shimizu T
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Journal Title
Immunity
Volume: 45
Issue: 4
Pages: 737-748
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of a Human Toll-Like Receptor (TLR) 8-Specific Agonist and a Functional Pan-TLR Inhibitor in 2-Aminoimidazoles.2016
Author(s)
Beesu, M., Caruso, G., Salyer, A. C., Shukla, N. M., Khetani, K. K., Smith, L. J., Fox, L. M., Tanji, H., Ohto, U., Shimizu, T., & David, S. A.
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Journal Title
J. Med. Chem.
Volume: 59
Issue: 7
Pages: 3311-3330
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Autoinhibition and relief mechanism by the proteolytic processing of Toll-like receptor 8.2016
Author(s)
Tanji, H., Ohto, U., Motoi, Y., Shibata, T., Miyake, K., & Shimizu, T.
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Journal Title
Proc. Natl. Acad. Sci. U S A
Volume: 113
Issue: 11
Pages: 3012-3017
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Structure-based Design of Human TLR8-specific Agonists with Augmented Potency and Adjuvanticity.2015
Author(s)
Beesu, M., Caruso, G., Salyer, A., Khetani, K., Sil, D., Weerasinghe, M., Tanji, H., Ohto, U., Shimizu, T., & David, S. A.
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Journal Title
J. Med. Chem.
Volume: 58
Issue: 19
Pages: 7833-7849
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The Imidazoquinoline Toll-Like Receptor-7/8 Agonist Hybrid-2 Potently Induces Cytokine Production by Human Newborn and Adult Leukocytes.2015
Author(s)
Ganapathi, L., Van Haren, S., Dowling, D. J., Bergelson, I., Shukla, N. M., Malladi, S. S., Balakrishna, R., Tanji, H., Ohto, U., Shimizu, T., David, S. A., & Levy, O.
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Journal Title
PLoS One
Volume: 10
Issue: 8
Pages: 0134640-0134640
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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