A new method for producing site-specific anti-protein antibodies at will
| Project/Area Number |
15K14472
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
三好 智博 新潟大学, 研究推進機構, 助教 (60534550)
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| Co-Investigator(Renkei-kenkyūsha) |
Sato Hiroe 新潟大学, 保健管理センター, 講師 (80705963)
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| Research Collaborator |
Suda Masahiro
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 生化学 / 自己抗体 / 自己免疫 / 抗P抗体 / リボソーム蛋白質 / 抗原性 / 免役化学 / 蛋白質 / 抗体 |
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| Outline of Final Research Achievements |
Antibodies are used in many research fields as useful tools. However, it is not so easy to produce antibodies that are useful for basic life science or clinical medicine in many cases. It has been suggested that the ribosomal P protein complex has unique structural features responsible for induction of anti-P autoantibody. In this study, I genetically added several kinds of amino acid sequences into a site of the P protein and immunized rabbits with the isolated chimeric proteins. As the results, I succeeded in producing site-specific antibodies, whose target sites were genetically induced to the P protein. So far, I detected productions of the following antibodies using this technique: antibodies to ribosomal protein S6, translation factor Hbs1, and Zucchine, an important factor involved in RNA interference. The results indicate that this technique could be used for productions of a variety of site-specific antibodies.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Characterization of anti-P monoclonal antibodies directed against the ribosomal protein•RNA complex antigen and produced using MRL autoimmune-prone mice.2015
Author(s)
Sato, H., Onozuka, M., Hagiya, A., Hoshino, S., Narita, I., and Uchiumi T.
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Journal Title
Clin. Exp. Immunol.
Volume: 179
Issue: 2
Pages: 236-244
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Functional role of the C-terminal tail of the archaeal ribosomal stalk in recruitment of two elongation factors to the sarcin/ricin loop of 23S rRNA.2015
Author(s)
Imai, H, Miyoshi, T, Murakami, R, Ito, K, Ishino, Y, and Uchiumi, T.
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Journal Title
Genes. Cells
Volume: 20
Issue: 7
Pages: 613-624
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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