Elucidation of a novel fatty acid transport system into mitochondria

• Project/Area Number: 15K14476
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Umeda Masato 京都大学, 工学研究科, 教授 (10185069)
• Co-Investigator(Renkei-kenkyūsha): NAGAO Kohjiro 京都大学, 大学院工学研究科, 助教 (40587325) ; JUNI Naoto 京都大学, 大学院工学研究科, 研究員 (90572199)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 脂質 / 脂肪酸 / ミトコンドリア / エネルギー代謝 / 筋肉

Outline of Final Research Achievements

Insects are the most abundant and diversified animal species on earth. Although the great diversity of form and function displayed by the insect is responsible for their superior adaptability to the changes in environmental conditions, the underlying molecular mechanisms remain elusive. Actively flying insects achieve the highest rates of energy metabolism known in the Animal Kingdom. However, the metabolic pathway that meets such a high-energy demand is not well understood. Here we demonstrate that fruit fly Drosophila melanogaster efficiently oxidizes fatty acids in the absence of carnitine palmitoyltransferase 1 (CPT1) that is indispensable for transporting long-chain fatty acids into mitochondria in mammalian cells. Genetic screen using cpt1-depleted Drosophila identified the five candidate genes that surrogated the CPT1-mediated fatty acid transport into mitochondria.

Research Products

• [Journal Article] 生体膜におけるリン脂質ダイナミクスとその生物機能 (2016)
  • Author(s): 土谷正樹・原雄二・梅田眞郷
  • Journal Title: 膜 Volume: 45 Pages: 196-201
  • NAID: 130005421182
• [Journal Article] ショウジョウバエのリン脂質輸送タンパク質－ユニークな形質膜のリン脂質の組成と分布 (2016)
  • Author(s): 長尾耕治郎・塩見晃史・梅田眞郷
  • Journal Title: 生体の化学 Volume: 67 Pages: 247-251
• [Journal Article] Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates (2015)
  • Author(s): Bhat HB, Ishitsuka R, Inaba T, Murate M, Abe M, Makino A, Kohyama-Koganeya A, Nagao K, Kurahashi A, Kishimoto T, Tahara M, Yamano A, Nagamune K, Hirabayashi Y, Juni N, Umeda M, Fujimori F, Nishibori K, Yamaji-Hasegawa A, Greimel P, Kobayashi T.
  • Journal Title: FASEB J Volume: 29(9) Issue: 9 Pages: 3920-34
  • DOI: 10.1096/fj.15-272112
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of a Novel Tetravalent Synthetic Peptide That Binds to Phosphatidic Acid. (2015)
  • Author(s): Rina Ogawa, Kohjiro Nagao, Kentaro Taniuchi, Masaki Tsuchiya, Utako Kato, Yuji Hara, Takehiko Inaba, Toshihide Kobayashi, Yoshihiro Sasaki, Kazunari Akiyoshi, Miho Watanabe-Takahashi, Kiyotaka Nishikawa, Masato Umeda
  • Journal Title: PLOS ONE Volume: 10 Issue: 7 Pages: e0131668-e0131668
  • DOI: 10.1371/journal.pone.0131668
  • NAID: 120005756258
  • Peer Reviewed / Open Access
• [Presentation] Dietary response governing thermoregulatory behavior (2016)
  • Author(s): Masato Umeda, Takuto Suito, Naoto Juni, Kojiro Nagao
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜）
  • Year and Date: 2016-11-30
• [Presentation] 腸内細菌を介する行動性体温調節の分子機構 (2016)
  • Author(s): 梅田眞郷、水藤拓人、従二直人、長尾耕治郎
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター(仙台）
  • Year and Date: 2016-09-25
• [Presentation] 体温はいかにして決まるのか？ (2016)
  • Author(s): 梅田眞郷
  • Organizer: HiHA第7回Workshop
  • Place of Presentation: 広島大学(広島）
  • Year and Date: 2016-09-23
• [Presentation] 温度センシングとエネルギー代謝 (2016)
  • Author(s): 梅田眞郷
  • Organizer: 第68回日本細胞生化学会大会
  • Place of Presentation: 早稲田大学西早稲田キャンパス(東京）
  • Year and Date: 2016-06-15
• [Presentation] 膜脂質ダイナミクスを介する細胞機能の制御機構 (2016)
  • Author(s): 梅田真郷
  • Organizer: 日本膜学会「第38年会」
  • Place of Presentation: 早稲田大学(東京）
  • Year and Date: 2016-05-10
  • Invited
• [Presentation] 脂質を介するエネルギー代謝と温度調節行動の制御機構 (2016)
  • Author(s): 梅田真郷
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター(札幌）
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] 生体膜脂質と温度感受性システム (2016)
  • Author(s): 梅田真郷
  • Organizer: 第36回白金シンポジウム
  • Place of Presentation: 北里大学薬学部(東京）
  • Year and Date: 2016-02-27
  • Invited
• [Presentation] 生体膜におけるリン脂質分子の運動と機能 (2015)
  • Author(s): 梅田真郷
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学(金沢）
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] 呼吸とエネルギー代謝：昆虫の秘密から学ぶ (2015)
  • Author(s): 梅田真郷
  • Organizer: 第19回酵素ダイナミクス研究会
  • Place of Presentation: 東京女子医科大学(東京）
  • Year and Date: 2015-09-12
  • Invited
• [Presentation] Asymmetric transbilayer distribution of membrane phospholipids is disrupted by constitutive activation of phospholipid scramblase in insect cells. (2015)
  • Author(s): Masato Umeda
  • Organizer: Lipids, Molecular & Cellular Biology of Gordon Research Conference
  • Place of Presentation: Waterville Valley（USA）
  • Year and Date: 2015-07-26
  • Int'l Joint Research
• [Presentation] 生物の温度応答と生体膜システムの関係 (2015)
  • Author(s): 梅田真郷
  • Organizer: 第59回生物医工学サロン
  • Place of Presentation: 京都大学再生医科学研究所(京都）
  • Year and Date: 2015-06-22
  • Invited
• [Presentation] 生物のロバスト制御と脂質生物学 (2015)
  • Author(s): 梅田真郷
  • Organizer: 第57回日本脂質生化学会
  • Place of Presentation: 一橋大学一橋講堂(東京）
  • Year and Date: 2015-05-29
  • Invited