Construction of photoregulation system of protein kinase by LOV domain
Project/Area Number |
15K14479
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
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Research Institution | Osaka Prefecture University |
Principal Investigator |
TOKUTOMI SATORU 大阪府立大学, 理学(系)研究科(研究院), 客員教授 (90142009)
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Co-Investigator(Renkei-kenkyūsha) |
IHARA HIDESHI 大阪府立大学, 大学院・理学系研究科, 准教授 (60254447)
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Research Collaborator |
KATSURA HITOMI 大阪府立大学, 大学院・理学系研究科, 研究員
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | フォトトロピン / 青色光受容体 / セリン/スレオニンキナーゼ / 活性光制御 / セリン/スレオニンキナーゼ |
Outline of Final Research Achievements |
Phototropin (phot) is a plant blue light receptor that mediates phototropic responses et al. Phot molecule has two photoreceptive domains named LOV1 and 2 in the N-terminal half and the C-terminus forms Ser/Thr kinase and acts as photoregulated protein kinase. It has been shown that conformational changes in Ja-helix residing between LOV2 and kinase domains play a crucial role in the kinase photoregulation. Mimicking this photoregulation mechanism, we tried to create a new photoregulation system of protein kinase A (PKA) using LOV2, Ja-helix and PKA inhibitor peptide (PKI). We succeeded in the light-induced prohibition of PKA by about 60% and also obtained new information regarding the molecular mechanism of the photoregulation. However, this was not applicable to ERK that is a key PKA in the MAP kinase pathway regulating cell growth and mitosis.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Pressure-sensitive Reaction Yield of the TePixD Blue-light Sensor Protein.2015
Author(s)
Kuroi, K., Okajima, K., Ikeuchi, M., Tokutomi, S., Kamiyama, T. and Terazima, M.
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Journal Title
J. Phys. Chem.
Volume: 119
Pages: 2897-2907
NAID
Related Report
Peer Reviewed
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[Journal Article] The effects of phytochrome-mediated light signals on the developmental acquisition of photoperiod sensitivity in rice.2014
Author(s)
Yoshitake, Y., Yokoo, T., Saito, H., Tsukiyama, T., Quan, Xu., Zikihara, K., Katsura, H., Tokutomi, S., Aboshi, T., Mori, N., Inoue, H., Nishida, H., Kohchi, T., Teraishi, M., Okumoto, Y. and Tanisaka, T.
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Journal Title
Sci. Rep.
Volume: 5
Issue: 1
Pages: 7709-7709
DOI
NAID
Related Report
Peer Reviewed / Open Access
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