Construction of photoregulation system of protein kinase by LOV domain

• Project/Area Number: 15K14479
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: TOKUTOMI SATORU 大阪府立大学, 理学(系)研究科(研究院), 客員教授 (90142009)
• Co-Investigator(Renkei-kenkyūsha): IHARA HIDESHI 大阪府立大学, 大学院・理学系研究科, 准教授 (60254447)
• Research Collaborator: KATSURA HITOMI 大阪府立大学, 大学院・理学系研究科, 研究員
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: フォトトロピン / 青色光受容体 / セリン/スレオニンキナーゼ / 活性光制御 / セリン／スレオニンキナーゼ

Outline of Final Research Achievements

Phototropin (phot) is a plant blue light receptor that mediates phototropic responses et al. Phot molecule has two photoreceptive domains named LOV1 and 2 in the N-terminal half and the C-terminus forms Ser/Thr kinase and acts as photoregulated protein kinase. It has been shown that conformational changes in Ja-helix residing between LOV2 and kinase domains play a crucial role in the kinase photoregulation. Mimicking this photoregulation mechanism, we tried to create a new photoregulation system of protein kinase A (PKA) using LOV2, Ja-helix and PKA inhibitor peptide (PKI). We succeeded in the light-induced prohibition of PKA by about 60% and also obtained new information regarding the molecular mechanism of the photoregulation. However, this was not applicable to ERK that is a key PKA in the MAP kinase pathway regulating cell growth and mitosis.

Research Products

• [Journal Article] Time-resolved fluctuation during the photochemical reaction of a photoreceptor protein: phototropin1LOV2-linker. (2016)
  • Author(s): Kuroi K, Sato F, Nakasone Y, Zikihara K, Tokutomi S, Terazima M.
  • Journal Title: Phys Chem Chem Phys. Volume: 18 Issue: 8 Pages: 6228-6238
  • DOI: 10.1039/c5cp07472j
  • NAID: 120005973369
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reconstitution of an initial step of phototropin signaling in stomatal guard cells (2016)
  • Author(s): Atsushi Takemiya, Ayaka Doi, Sayumi Yoshida, Koji Okajima, Satoru Tokutomi, Ken-ichiro Shimazaki
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 1 Pages: 152-159
  • DOI: 10.1093/pcp/pcv180
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The linker between LOV2-Ja and STK plays an essential role in the kinase activation by blue light in Arabidopsis phototropin1, a plant blue light receptor. (2015)
  • Author(s): Kashojiya, S., Yoshihara, S., Okajima, K. and Tokuotmi, S.
  • Journal Title: FEBS Lett. Volume: 590 Issue: 1 Pages: 139-147
  • DOI: 10.1002/1873-3468.12028
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Essential Role of the A'α/Aβ Gap in the N-Terminal Upstream of LOV2 for the Blue Light Signaling from LOV2 to Kinase in Arabidopsis Photototropin1, a Plant Blue Light Receptor. (2015)
  • Author(s): Kashojiya, S., Okajima, K., Shimada, T. and Tokutomi, S.
  • Journal Title: PLOS ONE Volume: 10 Issue: 4 Pages: e0124284-e0124284
  • DOI: 10.1371/journal.pone.0124284
  • Peer Reviewed / Open Access
• [Journal Article] Pressure-sensitive Reaction Yield of the TePixD Blue-light Sensor Protein. (2015)
  • Author(s): Kuroi, K., Okajima, K., Ikeuchi, M., Tokutomi, S., Kamiyama, T. and Terazima, M.
  • Journal Title: J. Phys. Chem. Volume: 119 Pages: 2897-2907
  • NAID: 120005973368
  • Peer Reviewed
• [Journal Article] From UV-B to Far-Red Light, a Variety of Plant Photoreceptors (2015)
  • Author(s): 徳富 哲、岡島公司、吉原静恵
  • Journal Title: Seibutsu Butsuri Volume: 55 Issue: 4 Pages: 181-186
  • DOI: 10.2142/biophys.55.181
  • NAID: 130005090036
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] The effects of phytochrome-mediated light signals on the developmental acquisition of photoperiod sensitivity in rice. (2014)
  • Author(s): Yoshitake, Y., Yokoo, T., Saito, H., Tsukiyama, T., Quan, Xu., Zikihara, K., Katsura, H., Tokutomi, S., Aboshi, T., Mori, N., Inoue, H., Nishida, H., Kohchi, T., Teraishi, M., Okumoto, Y. and Tanisaka, T.
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 7709-7709
  • DOI: 10.1038/srep07709
  • NAID: 120005758418
  • Peer Reviewed / Open Access
• [Presentation] Diversity of photo reactions of blue light sensor phototropins among different organisms (2017)
  • Author(s): Y.Nakasone, K.Okajima, K.Hitomi, J.Christie, S.Tokutomi, M.Terazima
  • Organizer: 新学術領域「動的秩序と機能」第５回国際シンポジウム
  • Place of Presentation: 東京都、東京大学 数理科学研究棟
  • Year and Date: 2017-01-21
• [Presentation] 圧力でタンパク質の揺らぎと反応を制御する:SyPixD (2016)
  • Author(s): 中島 翼、黒井邦巧、中曽根祐介、岡島公司、徳富 哲、寺嶋正秀
  • Organizer: 溶液化学研究会／第39回溶液化学シンポジウム
  • Place of Presentation: つくば市 産業技術総合研究所
  • Year and Date: 2016-11-09
  • Invited
• [Presentation] 青色光センサー全長フォトトロピンの光反応 (2016)
  • Author(s): 中曽根祐介、岡島公司、 相原悠介、長谷あきら、徳富哲、寺嶋正秀
  • Organizer: 第10回分子科学討論会
  • Place of Presentation: 神戸市 神戸ファッションマー
  • Year and Date: 2016-09-13
• [Presentation] 青色光センサータンパク質TePixDの高圧下における反応ダイナミクスの解析 (2016)
  • Author(s): 黒井邦巧、岡島公司、池内昌彦、徳富哲、猪股孝史、神山匡、寺嶋正秀
  • Organizer: 第 41 回生体分子科学討論会
  • Place of Presentation: 福岡市 九州大学西新プラザ
  • Year and Date: 2016-06-06
• [Presentation] phyC の短波長吸収特性は進化的に保存されている (2016)
  • Author(s): 吉原 静恵，石黒 志保，田中 大樹，大久保 陽子，加川 貴俊，西塚 順子，岡島 公司，徳富 哲
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学、盛岡
  • Year and Date: 2016-03-18
• [Presentation] ロイヌナズナPIF3はphyBのN-末端延長配列に結合して暗回帰反応を阻害する (2016)
  • Author(s): 桂ひとみ，直原一徳，松下智直，吉原静恵，河内孝之，長谷あきら，德富哲
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学、盛岡
  • Year and Date: 2016-03-18
• [Presentation] Phytochrome-Interacting Factor 3 binds to N-terminal extension of Arabidopsis phytochrome B and prohibit dark reversion. (2015)
  • Author(s): Satoru Tokutomi
  • Organizer: 7th Asia and Oceania Conference for Photobiology
  • Place of Presentation: Academia Sinica, Taipei, Taiwan
  • Year and Date: 2015-11-15
  • Int'l Joint Research / Invited
• [Presentation] Phototropin, a blue light-regulated protein kinase in plants. (2015)
  • Author(s): Satoru Tokutomi
  • Organizer: 16th Congress of the European for Photobiology
  • Place of Presentation: Aveiro Univ. Aveiro, Portugal.
  • Year and Date: 2015-08-31
  • Int'l Joint Research / Invited