Development of the photoswitch systems sensing rainbow colors

• Project/Area Number: 15K14486
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Toyohashi University of Technology
• Principal Investigator: Hirose Yuu 豊橋技術科学大学, 工学(系)研究科(研究院), 助教 (30616230)
• Research Collaborator: EKI Toshihiko 豊橋技術科学大学, 環境・生命工学系・大学院工学研究科, 教授 (40192512)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 次世代シークエンサー / フィトクロム / オプトジェネティクス / シアノバクテリア / 光受容体 / 二成分制御系 / シアノバクテリオクロム / 光スイッチ / 合成生物学

Outline of Final Research Achievements

In this study, we aimed to develop the photoswitch systems sensing rainbow colors by introducing random mutagenesis in the cyanobacteriochrome-class photosensor and by its screening in E. coli using next generation sequencers. We introduced the photoswitch system and light regulated promoter fused to antibiotics genes in E. coli, but did not observe clear control of its growth rate by light irradiation. Further analyses suggested that 1) activation of light-regulated promoter by E. coli transcriptional system and 2) inefficient transcriptional activation probably due to low efficiency in chromophore attachment of photosensor protein and/or its low phosphorylation activity. Therefore, we are now trying a superior photoswitch identified in different host cyanobacteria and establishing more systematic approach.

Research Products

• [Journal Article] Characterization of the Genuine Type 2 Chromatic Acclimation in the Two Geminocystis Cyanobacteria. (2017)
  • Author(s): Hirose Y., Misawa N., Yonekawa C., Nagao N., Watanabe M., Ikeuchi M. and Eki T.
  • Journal Title: DNA Res. Volume: 5(14) Issue: 4 Pages: 387-396
  • DOI: 10.1093/dnares/dsx011
  • Peer Reviewed / Open Access
• [Journal Article] Diverse molecular processes of chromatic acclimation in the cyanobacteria phylum. (2017)
  • Author(s): Hirose Y., Ikeuchi M. and Eki T.
  • Journal Title: Plant Morphol. Volume: 印刷中
  • NAID: 130006647120
  • Peer Reviewed / Open Access
• [Journal Article] Complete genome sequence of cyanobacterium Nostoc sp. NIES-3756, a potentially useful strain for phytochrome-based bioengineering. (2016)
  • Author(s): Hirose Y., Fujisawa T., Ohtsubo Y., Katayama M., Misawa N., Wakazuki S., Shimura Y., Nakamura Y., Kawachi M., Yoshikawa H., Eki T., and Kanesaki Y.
  • Journal Title: Journal of Biotechnology Volume: 220 Pages: 51-52
  • DOI: 10.1016/j.jbiotec.2015.12.002
  • Peer Reviewed / Int'l Joint Research
• [Presentation] シアノバクテリアの光スイッチの改良 (2016)
  • Author(s): 広瀬侑
  • Organizer: 第１０回長野セミナー
  • Place of Presentation: ラフォーレ倶楽部白馬八方
  • Year and Date: 2016-01-24
• [Patent(Industrial Property Rights)] 核酸分子およびこれを用いたタンパク質の製造方法 (2017)
  • Inventor(s): 広瀬侑
  • Industrial Property Rights Holder: 広瀬侑
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017-03-01