| Project/Area Number |
15K14487
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Nagai Takeharu 大阪大学, 産業科学研究所, 教授 (20311350)
|
|---|
| Research Collaborator |
Brooker Gary Johns Hopkins大学, Biomedical Engineering, 教授
TAHARA ITSUKI 関西大学, システム理工学部, 助教 (50709095)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | 化学発光 / ホログラフィー / 超解像顕微鏡 / イメージング / ３次元 |
|---|
| Outline of Final Research Achievements |
We proceeded experiment toward development of “Superresolution chemiluminescence holographic microscopy” enabling chemiluminescence image acquisition with high speed, three-dimension and superresolution quality, by applying chemiluminescent protein with Fresnel incoherent correlation holography (FINCH) and wavefront correction system. By observing HeLa cells expressing chemiluminrescent protein “Nano-lantern” using FINCH, we could reconstruct the chemiluminescent tomographic images with as good quality as the fluorescence ones. To correct wavefront distortion of light, we constructed a wavefront correction system and evaluated the superior performance. To achieve high speed and multi-color acquisition of the chemiluminescent holograms, we developed color palettes of chemiluminescent protein “eNano-lantern” with approximately 10-fold improvement in the brightness compared with previous Nano-lantern.
|
