Development of a method for real-time measuring ion concentrations within membrane contact sites
| Project/Area Number |
15K14488
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
MAEDA YUSUKE 大阪大学, 微生物病研究所, 准教授 (00294124)
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| Project Period (FY) |
2015-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 膜接触部位 / pH / カルシウム / バイオイメージング / カルシウムセンサー / pHセンサー |
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| Outline of Final Research Achievements |
To reveal the communications between organelles through membrane contact sites (MCS), we established the assay system to measure ion concentrations within the MCS in real-time as the following. First, we created organelle-specifically localized reporter molecules harboring FRB or FKBP, and these molecules could form MCS by adding rapamycin. Taken pH-resistance into consideration, we created a fusion molecule of GFP and aequorin as a calcium sensor. By fusing organelle-specific reporter molecules with ion-sensitive fluorescent reporter molecules such as pHluorin and calcium sensor, assay systems for ion measurement in MCS were established.
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Report
(2 results)
Research Products
(3 results)
