Model biological membrane having a nanometric gap structure

• Project/Area Number: 15K14489
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Kobe University
• Principal Investigator: Morigaki Kenichi 神戸大学, バイオシグナル総合研究センター, 准教授 (10358179)
• Co-Investigator(Renkei-kenkyūsha): Hayashi Fumio 神戸大学, 大学院理学系研究科, 名誉教授 (80093524)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 膜タンパク質 / 人工膜 / ナノ空間 / 1分子計測 / 生体膜 / ナノ流路

Outline of Final Research Achievements

We developed a model biological membrane having a nanometric gap structure (nanogap-junction) as a platform for the single molecule observation of membrane proteins. The model biological membrane was composed of polymeric and fluid lipid bilayers. The polymeric lipid bilayer was attached with a silicone elastomer (PDMS) sheet using polymer-brush-coated silica nanoparticles as the adhesion layer, creating a nanometric gap junction between the fluid bilayer and PDMS. Silica nanoparticles proved to be an excellent material for the adhesion layer due to the mono-disperse size and mechanical stability. Thickness of the nanogap-junction was controlled by the size of silica nanoparticles. Nanogap-junction enabled to observe reconstituted membrane proteins with a heightened signal-to-noise (S/N) ratio. The nanogap-junction provides new opportunities for the single molecule biophysics of membrane proteins.

Research Products

• [Journal Article] Evolution and development of model membranes for physicochemical and functional studies of the membrane lateral heterogeneity (2018)
  • Author(s): Morigaki Kenichi、Tanimoto Yasushi
  • Journal Title: Biochim Biophys Acta. Volume: 印刷中 Issue: 10 Pages: 2012-2017
  • DOI: 10.1016/j.bbamem.2018.03.010
  • Peer Reviewed
• [Journal Article] Hybrid Model Membrane Combining Micropatterned Lipid Bilayer and Hydrophilic Polymer Brush (2017)
  • Author(s): Nishimura Toshiki、Tamura Fuyuko、Kobayashi Sawako、Tanimoto Yasushi、Hayashi Fumio、Sudo Yuki、Iwasaki Yasuhiko、Morigaki Kenichi
  • Journal Title: Langmuir Volume: 33 Issue: 23 Pages: 5752-5759
  • DOI: 10.1021/acs.langmuir.7b00463
  • Peer Reviewed
• [Journal Article] The role of the Prod1 membrane anchor in newt limb regeneration (2017)
  • Author(s): Nomura, K., Tanimoto, Y., Hayashi, F., Harada, E., Shan, X.-Y., Shionyu, M., Hijikata, A., Shirai, T., Morigaki, K., Shimamoto, K.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 56 Issue: 1 Pages: 270-274
  • DOI: 10.1002/anie.201609703
  • Peer Reviewed / Open Access
• [Journal Article] Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs (2017)
  • Author(s): Masanao Kinoshita, Kenichi GN Suzuki, Nobuaki Matsumori, Misa Takada, Hikaru Ano, Kenichi Morigaki, Mitsuhiro Abe, Asami Makino, Toshihide Kobayashi, Koichiro M Hirosawa, Takahiro K Fujiwara, Akihiro Kusumi, Michio Murata
  • Journal Title: J Cell Biol Volume: 未定 Issue: 4 Pages: 1183-1204
  • DOI: 10.1083/jcb.201607086
  • NAID: 120005998045
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Nanometric gap structure with fluid lipid bilayer for the selective transport and detection of biological molecules (2016)
  • Author(s): Ando, K., Tanabe, M., Morigaki, K.
  • Journal Title: Langmuir Volume: 32 Issue: 31 Pages: 7958-7964
  • DOI: 10.1021/acs.langmuir.6b01405
  • Peer Reviewed
• [Journal Article] パターン化脂質膜を基盤とした人工生体膜の創出 (2016)
  • Author(s): 5)森垣憲一
  • Journal Title: 表面科学 Volume: 37 Pages: 230-234
  • NAID: 130005154320
  • Peer Reviewed
• [Journal Article] Evaluating the raftophilicity of rhodopsin photoreceptor in a patterned model membrane (2015)
  • Author(s): Tanimoto, Y., Okada, K., *Hayashi, F., Morigaki, K.
  • Journal Title: Biophysical Journal Volume: 109 Issue: 11 Pages: 2307-2316
  • DOI: 10.1016/j.bpj.2015.10.015
  • Peer Reviewed
• [Presentation] Patterned model biological membrane on the solid substrate (2018)
  • Author(s): Morigaki, K.
  • Organizer: 9th International Workshop on Nanostructures and Nanoelectronics
  • Int'l Joint Research / Invited
• [Presentation] Nanometric gap structure for selective biosensing created with patterned lipid bilayer, silicone elastomer, and silica nanoparticles (2018)
  • Author(s): Morigaki, K., Tanabe, M., Komatsu, R., Ando, K.
  • Organizer: 255th American Chemical Society National Meeting
  • Int'l Joint Research / Invited
• [Presentation] Nanogap-junction with a fluid lipid bilayer for selective biosensing (2017)
  • Author(s): Morigaki, K.
  • Organizer: 8th International Workshop on Nanostructures & Nanoelectronics
  • Place of Presentation: Tohoku University
  • Year and Date: 2017-03-06
  • Int'l Joint Research / Invited
• [Presentation] Hybrid model membrane combining micropatterned lipid bilayer and hydrophilic polymer brush (2017)
  • Author(s): Morigaki, K., Nishimura, T., Tamura, F., Kobayashi, S., Tanimoto, Y., Sudo, Y., Hayashi, F., Iwasaki, K.
  • Organizer: 253rd American Chemical Society National Meeting
  • Int'l Joint Research / Invited
• [Presentation] 人工膜を用いた膜シグナル伝達機構の再構成と定量的解析 (2017)
  • Author(s): 森垣憲一
  • Organizer: 理研シンポジウム「細胞システムの動態と論理 IX」
  • Invited
• [Presentation] Micropatterned model membrane to elucidate the functional roles of lipid micro-domains in the signal transduction cascade (2017)
  • Author(s): Morigaki, K.
  • Organizer: Tethmem 2017
  • Int'l Joint Research / Invited
• [Presentation] Model membrane on a solid substrate: Crawling to grow from 2D into 3D structures (2017)
  • Author(s): Morigaki, K.
  • Organizer: BioNano summer school 2017
  • Int'l Joint Research / Invited
• [Presentation] Model membrane on the solid substrates with increased complexity and functions (2017)
  • Author(s): Morigaki, K.
  • Organizer: International Symposium on Science and Technology of Molecular Assemblies for Biomaterials Innovation
  • Int'l Joint Research / Invited
• [Presentation] Patterned lipid bilayer on solid substrate as a versatile model system of biological membrane (2017)
  • Author(s): Morigaki, K.
  • Organizer: 3rd International Conference on Bioinspired and Zwitterionic Materials
  • Int'l Joint Research / Invited
• [Presentation] Reconstitution and quantitative analyses of membrane signal transduction by using micropatterned model system (2017)
  • Author(s): Morigaki, K., Tanimoto, Y., Kojima, S., Awazu, A., Hayashi, F.
  • Organizer: ConBio 2017
  • Invited
• [Presentation] Evaluating the raftophilicity of rhodopsin photoreceptor in a patterned model membrane (2016)
  • Author(s): Morigaki, K., Tanimoto, Y., Hayashi, F
  • Organizer: 251st American Chemical Society National Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-03-13
  • Int'l Joint Research / Invited
• [Presentation] パターン化脂質膜とナノ空間を融合した新規人工生体膜の創出 (2015)
  • Author(s): 森垣憲一，安藤公二，田邊真志
  • Organizer: 第 35回表面科学学術講演会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Micro-/ nano-compartments between substrate-supported model membrane and silicone elastomer (2015)
  • Author(s): Morigaki, K.
  • Organizer: TethMem 2015
  • Place of Presentation: Nagyan University, Singapore
  • Year and Date: 2015-11-08
  • Int'l Joint Research / Invited
• [Presentation] Patterned lipid bilayer on solid substrate as a versatile model system of the biological membrane (2015)
  • Author(s): Morigaki, K.
  • Organizer: Biological Research Centre, Hungarian Academy of Science
  • Place of Presentation: Szeged, Hungary
  • Year and Date: 2015-08-31
  • Int'l Joint Research / Invited
• [Remarks] 神戸大学 環境物質科学
  • URL: http://www.research.kobe-u.ac.jp/rceg-nowstone/index.html
• [Remarks] 神戸大学バイオシグナル総合研究センター環境物質科学研究室
  • URL: http://www.research.kobe-u.ac.jp/rceg-nowstone/index.html
• [Remarks] 神戸大学 環境物質科学研究室
  • URL: http://www.research.kobe-u.ac.jp/rceg-nowstone/index.html