| Project/Area Number |
15K14489
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Kobe University |
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Principal Investigator |
Morigaki Kenichi 神戸大学, バイオシグナル総合研究センター, 准教授 (10358179)
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| Co-Investigator(Renkei-kenkyūsha) |
Hayashi Fumio 神戸大学, 大学院理学系研究科, 名誉教授 (80093524)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 膜タンパク質 / 人工膜 / ナノ空間 / 1分子計測 / 生体膜 / ナノ流路 |
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| Outline of Final Research Achievements |
We developed a model biological membrane having a nanometric gap structure (nanogap-junction) as a platform for the single molecule observation of membrane proteins. The model biological membrane was composed of polymeric and fluid lipid bilayers. The polymeric lipid bilayer was attached with a silicone elastomer (PDMS) sheet using polymer-brush-coated silica nanoparticles as the adhesion layer, creating a nanometric gap junction between the fluid bilayer and PDMS. Silica nanoparticles proved to be an excellent material for the adhesion layer due to the mono-disperse size and mechanical stability. Thickness of the nanogap-junction was controlled by the size of silica nanoparticles. Nanogap-junction enabled to observe reconstituted membrane proteins with a heightened signal-to-noise (S/N) ratio. The nanogap-junction provides new opportunities for the single molecule biophysics of membrane proteins.
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