| Project/Area Number |
15K14490
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Yoshikane Kunihiro 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (20467616)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUKAZAKI Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
KONNO Hiroki 金沢大学, バイオAFM先端研究センター, 准教授 (80419267)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 蛋白質 / タンパク質 |
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| Outline of Final Research Achievements |
Protein secretion machinery transports precursor proteins across membranes with dynamic structural changes. Although the detailed structures of each factor have been reported by X-ray crystallography, it is still unknown how the factors undergo conformational transitions. In this study, we established a new reconstruction system using nanodiscs and observed the nanodiscs by high speed AFM. It is time to analyze the protein secretion using this tool.
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