Construction of a new reconstruction system and analysis of dynamic protein secretion machinery
Project/Area Number |
15K14490
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Yoshikane Kunihiro 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (20467616)
|
Co-Investigator(Renkei-kenkyūsha) |
TSUKAZAKI Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
KONNO Hiroki 金沢大学, バイオAFM先端研究センター, 准教授 (80419267)
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Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 蛋白質 / タンパク質 |
Outline of Final Research Achievements |
Protein secretion machinery transports precursor proteins across membranes with dynamic structural changes. Although the detailed structures of each factor have been reported by X-ray crystallography, it is still unknown how the factors undergo conformational transitions. In this study, we established a new reconstruction system using nanodiscs and observed the nanodiscs by high speed AFM. It is time to analyze the protein secretion using this tool.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Tunnel formation inferred from the I form structures of the proton-driven protein secretion motor SecDF2017
Author(s)
Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
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Journal Title
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Snapshots of the proton-driven protein translocation motor2017
Author(s)
Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
Organizer
EMBO conference | Protein translocation and cellular homeostasis
Place of Presentation
Hotel Valamar Lacroma (クロアチア・ドブロブニク)
Year and Date
2017-03-18
Related Report
Int'l Joint Research / Invited
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[Presentation] タンパク質膜透過を駆動するモータータンパク質のスナップショット2017
Author(s)
古川 新, 吉海江 国仁, 森 貴治, 森 博幸, 森本 雄祐, 菅野 泰功, 岩木 薫大, 南野 徹, 杉田 有治, 田中 良樹, 塚崎 智也
Organizer
2017年 生体運動研究合同班会議
Place of Presentation
神戸国際会議場(兵庫県・神戸市)
Year and Date
2017-01-06
Related Report
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[Presentation] Snapshots of a protein translocation motor2016
Author(s)
古川 新, 吉海江 国仁, 森 貴治, 森 博幸, 森本 雄祐, 菅野 泰功, 岩木 薫大, 南野 徹, 杉田 有治, 田中 良樹, 塚崎 智也
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県・つくば市)
Year and Date
2016-11-25
Related Report
Int'l Joint Research / Invited
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[Presentation] Snapshots of a proton-driven protein translocation motor2016
Author(s)
Tomoya Tsukazaki, Kunihito Yoshikaie, Arata Furukawa, Yasunori Sugano, Yoshiki Tanaka
Organizer
The 42nd Naito Conference on In the Vanguard of Structural Biology: Revolutionizing Life Sciences
Place of Presentation
シャトレーゼ ガトーキングダム サッポロ(北海道・札幌市)
Year and Date
2016-10-04
Related Report
Int'l Joint Research / Invited
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[Presentation] 新生鎖を膜透過させるSec蛋白質のX線結晶構造解析2016
Author(s)
田中 良樹, 菅野 泰功, 武本 瑞貴, 森 貴治, 古川 新, 吉海江 国仁, 草木迫 司, 熊崎 薫, 鹿島 絢子, 石谷 隆一郎, 杉田 有治, 濡木 理, 塚崎 智也
Organizer
第16回日本蛋白質科学会 年会
Place of Presentation
福岡国際会議場(福岡県・福岡市)
Year and Date
2016-06-06
Related Report
Invited
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