| Project/Area Number |
15K14491
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HORIKAWA Kazuki 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (70420247)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | イメージング / 生物リズム / 自己組織化 / cAMP / 離散系 / 興奮系 / バイオメージング / 一分子計測 / シグナル伝達 |
|---|
| Outline of Final Research Achievements |
To ask the mechanisms in the construction of oscillatory signaling circuit, we performed single molecule quantification for essential components in the cAMP signaling system. We developed essential technologies including the efficient generation of GFP-tagged cell lines(BMC Biotech, 2016) and ultrasensitive cAMP indicators (ACS Chem Biol, 2016). By utilizing these tools, we performed a simultaneous imaging of cellular function (cAMP) and molecular quantification and identified that self-organized construction of signaling circuit is regulated by few proteins characterized with significantly low level expression.
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