• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Single molecule analysis in the generation of oscillatory circuit for cAMP signaling

Research Project

Project/Area Number 15K14491
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionThe University of Tokushima

Principal Investigator

HORIKAWA Kazuki  徳島大学, 大学院医歯薬学研究部(医学系), 教授 (70420247)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsイメージング / 生物リズム / 自己組織化 / cAMP / 離散系 / 興奮系 / バイオメージング / 一分子計測 / シグナル伝達
Outline of Final Research Achievements

To ask the mechanisms in the construction of oscillatory signaling circuit, we performed single molecule quantification for essential components in the cAMP signaling system. We developed essential technologies including the efficient generation of GFP-tagged cell lines(BMC Biotech, 2016) and ultrasensitive cAMP indicators (ACS Chem Biol, 2016). By utilizing these tools, we performed a simultaneous imaging of cellular function (cAMP) and molecular quantification and identified that self-organized construction of signaling circuit is regulated by few proteins characterized with significantly low level expression.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Nontrivial Effect of the Color-Exchange of a Donor/Acceptor Pair in the Engineering of Forster Resonance Energy Transfer (FRET)-Based Indicators.2016

    • Author(s)
      Yusaku Ohta, Takanori Kamagata, Asuka Mukai, Shinji Takada, Takeharu Nagai, and Kazuki Horikawa
    • Journal Title

      ACS Chemical Biology

      Volume: 11(7) Issue: 7 Pages: 1816-1822

    • DOI

      10.1021/acschembio.6b00221

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum2016

    • Author(s)
      Asuka Mukai, Aya Ichiraku, Kzuki Horikawa
    • Journal Title

      BMC Biotechnology

      Volume: なし Issue: 1 Pages: 37-37

    • DOI

      10.1186/s12896-016-0267-8

    • NAID

      120006814108

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effective impairment of myeloma cells and their progenitors by blockade of monocarboxylate transportation2015

    • Author(s)
      6. Hanson DJ, Nakamura S, Amachi R, Hiasa M, Oda A, Tsuji D, Itoh K, Harada T, Horikawa K, Teramachi J, Miki H, Matsumoto T, Abe M.
    • Journal Title

      Oncotarget

      Volume: 6 Issue: 32 Pages: 33568-33586

    • DOI

      10.18632/oncotarget.5598

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Minority control of synchronized dynamics in biological oscillator2015

    • Author(s)
      堀川一樹
    • Organizer
      第38回日本分子生物学会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 自発的発振回路の新規構築原理2015

    • Author(s)
      堀川一樹
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2015-05-13
    • Related Report
      2015 Research-status Report
  • [Book] 少数性生物学2017

    • Author(s)
      永井健治、村越秀治、大場雄介、富樫祐一、小松﨑民樹、城口克之、野地博行、前島一博、粟津暁紀、鈴木宏明、茅 元司、矢島潤一郎、谷口雄一、小嶋誠司、今田勝巳、石島秋彦、福岡 創、蔡 栄淑、堀川一樹、大出晃士
    • Total Pages
      178
    • Publisher
      日本評論社
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi