Control of cell differentiation by the cytoplasmic pH change

• Project/Area Number: 15K14498
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Morimoto Yusuke 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50631777)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: シグナル伝達 / 細部内pH / 蛍光イメージング / 細胞分化 / 光遺伝学 / 生物物理 / 細胞内pH

Outline of Final Research Achievements

Intracellular pH plays important roles in the signal transduction for cell differentiation. However it remains unknown how intracellular pH change works in the cell differentiation. To investigate the role of intracellular pH, we constructed Dictyostelium discoideum strain expressing pH-sensitive fluorescent protein and observed cytoplasmic pH changes in the multicellular stage by fluorescence microscopy. And we could regulate the cytoplasmic pH of Dictyostelium cells expressing channelrhodopsin by photostimulation. We will try to control the cell differentiation by photostimulation.

Research Products

• [Journal Article] Measurements of free-swimming speed of motile Salmonella cells in liquid media (2017)
  • Author(s): Yusuke V. Morimoto, Keiichi Namba, Tohru Minamino
  • Journal Title: Bio-protocol Volume: 7 Issue: 1
  • DOI: 10.21769/bioprotoc.2093
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bacterial intracellular sodium ion measurement using CoroNa Green (2017)
  • Author(s): Yusuke V. Morimoto, Keiichi Namba, Tohru Minamino
  • Journal Title: Bio-protocol Volume: 7 Issue: 1
  • DOI: 10.21769/bioprotoc.2092
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Stoichiometry and turnover of the stator and rotor (2017)
  • Author(s): Yusuke V. Morimoto, Tohru Minamino
  • Journal Title: Methods in Molecular Biology Volume: 1593 Pages: 203-213
  • DOI: 10.1007/978-1-4939-6927-2_16
  • ISBN: 9781493969265, 9781493969272
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Straight and rigid flagellar hook made by insertion of the FlgG specific sequence into FlgE (2017)
  • Author(s): Koichi D. Hiraoka, Yusuke V. Morimoto, Yumi Inoue, Takashi Fujii, Tomoko Miyata, Fumiaki Makino, Tohru Minamino, Keiichi Namba
  • Journal Title: Scientific reports Volume: 7 Issue: 1 Pages: 46723-46723
  • DOI: 10.1038/srep46723
  • Peer Reviewed / Open Access
• [Journal Article] Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF (2017)
  • Author(s): Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
  • Journal Title: Cell Reports Volume: 19 Issue: 5 Pages: 1-7
  • DOI: 10.1016/j.celrep.2017.04.030
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High-resolution pH imaging of living bacterial cell to detect local pH differences (2016)
  • Author(s): Yusuke V. Morimoto, Nobunori Kami-ike, Tomoko Miyata, Akihiro Kawamoto, Takayuki Kato, Keiichi Namba, Tohru Minamino
  • Journal Title: mBio Volume: 7 Issue: 6
  • DOI: 10.1128/mbio.01911-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The bacterial flagellar type III export gate complex is a dual fuel engine that can use both H+ and Na+ for flagellar protein export (2016)
  • Author(s): Minamino T, Morimoto YV, Hara N, Aldridge PD, Namba K
  • Journal Title: PLoS Pathog Volume: 12 Issue: 3 Pages: e1005495-e1005495
  • DOI: 10.1371/journal.ppat.1005495
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella. (2016)
  • Author(s): Minamino T, Kinoshita M, Inoue Y, Morimoto YV, Ihara K, Koya S, Hara N, Nishioka N, Kojima S, Homma M, Namba K
  • Journal Title: Microbiologyopen Volume: - Issue: 3 Pages: 340-340
  • DOI: 10.1002/mbo3.340
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] H+ and Na+ are involved in flagellar rotation of the spirochete Leptospira. (2015)
  • Author(s): Islam MS, Morimoto YV, Kudo S, Nakamura S
  • Journal Title: Biochem Biophys Res Commun Volume: 466 Issue: 2 Pages: 196-200
  • DOI: 10.1016/j.bbrc.2015.09.004
  • Peer Reviewed
• [Presentation] 細胞の集団運動に関わる電気化学ポテンシャル変化の 高感度イメージング (2017)
  • Author(s): 森本雄祐、上田昌宏
  • Organizer: 日本物理学会第72 回年次大会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2017-03-18
  • Invited
• [Presentation] DETECTION OF LOCAL PH DIFFERENCES IN LIVING BACTERIAL CELL BY HIGH-RESOLUTION PH IMAGING (2017)
  • Author(s): Yusuke V. Morimoto, Nobunori Kami-ike, Tomoko Miyata, Akihiro Kawamoto, Takayuki Kato, Keiichi Namba, Tohru Minamino
  • Organizer: BLAST XIV
  • Place of Presentation: Royal Sonesta Hotel (New Orleans, USA)
  • Year and Date: 2017-01-16
  • Int'l Joint Research
• [Presentation] 細胞機能に関わる細胞内pHの計測 (2016)
  • Author(s): 森本雄祐、上田昌宏
  • Organizer: 第５４回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-26
  • Invited
• [Presentation] cAMPシグナルリレーにおける膜電位変化の計測と制御, 第５４回日本生物物理学会年会 (2016)
  • Author(s): 森本雄祐、上田昌宏
  • Organizer: 第５４回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-26
• [Presentation] 細胞性粘菌における膜電位の計測と制御 (2016)
  • Author(s): 森本雄祐、上田昌宏
  • Organizer: 日本細胞性粘菌学会第６回例会
  • Place of Presentation: 上智大学
  • Year and Date: 2016-10-15
• [Presentation] バクテリア１細胞内における局所pH差の計測 (2016)
  • Author(s): 森本雄祐, 上池伸徳, 宮田知子, 川本晃大, 加藤貴之, 難波啓一, 南野徹
  • Organizer: 研究会「理論と実験」２０１６
  • Place of Presentation: 広島大学
  • Year and Date: 2016-10-07
• [Presentation] 協調的アメーバ運動を司る局所的膜電位ゆらぎの計測 (2016)
  • Author(s): 森本雄祐、上田昌宏
  • Organizer: 新学術領域「運動超分子マシナリーが織りなす調和と多様性」第４回領域全体会議
  • Place of Presentation: 長崎大学良順会館
  • Year and Date: 2016-06-10
• [Presentation] 単細胞・多細胞における高感度細胞内シグナルイメージング (2016)
  • Author(s): 森本雄祐、上田昌宏
  • Organizer: 新学術領域「運動超分子マシナリーが織りなす調和と多様性」第４回領域全体会議
  • Place of Presentation: 長崎大学良順会館
  • Year and Date: 2016-06-08
• [Presentation] 細胞性粘菌における多細胞動態イメージング (2016)
  • Author(s): 森本雄祐
  • Organizer: The 2nd RIKEN Quantitative and Developmental Biology Joint Workshop
  • Place of Presentation: 理化学研究所（神戸キャンパス）
  • Year and Date: 2016-04-19
  • Invited
• [Presentation] 走化性シグナル伝達に伴う自発的膜電位変化 (2016)
  • Author(s): 森本雄祐
  • Organizer: 理研シンポジウム「細胞システムの動態と論理VIII」
  • Place of Presentation: 理化学研究所（和光キャンパス）
  • Year and Date: 2016-04-15
• [Presentation] 自発的膜電位変化を伴う走化性シグナル伝達 (2016)
  • Author(s): 森本雄祐，上田昌宏
  • Organizer: 2016年 生体運動合同班会議
  • Place of Presentation: キャンパスプラザ京都（京都府京都市）
  • Year and Date: 2016-01-09
• [Presentation] 細胞性粘菌におけるシグナル伝達と膜電位変化の関係 (2015)
  • Author(s): 森本雄祐，上田昌宏
  • Organizer: 第５回日本細胞性粘菌学会例会
  • Place of Presentation: 弘前大学（青森県弘前市）
  • Year and Date: 2015-10-11
• [Presentation] High-sensitivity fluorescence imaging of membrane potential in Dictyostelium (2015)
  • Author(s): 森本雄祐，上田昌宏
  • Organizer: 第５３回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-15
• [Presentation] 細胞内局所pHと細胞機能の関係 (2015)
  • Author(s): 森本雄祐，上田昌宏
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2015-04-20
• [Remarks] 細胞内生体ナノマシン近傍のpH計測が可能に
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/events/achievement/namba-20161206/