Control of cell differentiation by the cytoplasmic pH change
Project/Area Number |
15K14498
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biophysics
|
Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Morimoto Yusuke 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50631777)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | シグナル伝達 / 細部内pH / 蛍光イメージング / 細胞分化 / 光遺伝学 / 生物物理 / 細胞内pH |
Outline of Final Research Achievements |
Intracellular pH plays important roles in the signal transduction for cell differentiation. However it remains unknown how intracellular pH change works in the cell differentiation. To investigate the role of intracellular pH, we constructed Dictyostelium discoideum strain expressing pH-sensitive fluorescent protein and observed cytoplasmic pH changes in the multicellular stage by fluorescence microscopy. And we could regulate the cytoplasmic pH of Dictyostelium cells expressing channelrhodopsin by photostimulation. We will try to control the cell differentiation by photostimulation.
|
Report
(3 results)
Research Products
(24 results)
-
-
-
-
-
[Journal Article] Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF2017
Author(s)
Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
-
Journal Title
Cell Reports
Volume: 19
Issue: 5
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
[Journal Article] FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.2016
Author(s)
Minamino T, Kinoshita M, Inoue Y, Morimoto YV, Ihara K, Koya S, Hara N, Nishioka N, Kojima S, Homma M, Namba K
-
Journal Title
Microbiologyopen
Volume: -
Issue: 3
Pages: 340-340
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-