Development of fluorescence labeling methods of proteins in cells and tissues for high-resolution correlated light-electron microscopy (CLEM) using Atmospheric scanning electron microscopy
| Project/Area Number |
15K14499
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SATO Chikara 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (00357146)
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| Co-Investigator(Kenkyū-buntansha) |
海老原 達彦 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ付 (00344119)
岡田 知子 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (30344146)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 電子顕微鏡 / バイオイメージング / 電子線励起 / 光電子相関顕微鏡 / 転移性がん細胞 |
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| Outline of Final Research Achievements |
Proteins associate with other proteins and form protein complexes, localizing in a specific positions in cells and tissues for their functions. The correlative light-electron microscopy (CLEM) of a sample in liquid is highly required to determine their localization at high resolution. To realize electron microscopy in solution, environmental-capsule electron microscopy (EC-EM) has been developed. However, the limited space around the sample holder of standard TEM, preclude the simultaneous observation using OM with high resolution objective lens of large NA. We have developed atmospheric scanning electron microscopy (ASEM) with inverted SEM to allow the use of an open sample container sandwiched by OM and SEM. In this research, we have tested many fluorescent substances, including GFP, qDot and ZnO. Further the markers tested here should be exploited for the application to various biological fields including basic biology, medical research, agriculture, drug discovery and diagnosis.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Mice lacking the intracellular cation channel TRIC-B have compromised collagen production and impaired bone mineralization.2016
Author(s)
C.Zhao, A.Ichimura, N.Qian, T.Iida, D.Yamazaki, N.Noma, M.Asagiri, K.Yamamoto, S.Komazaki, C.Sato, F.Aoyama, A.Sawaguchi, S.Kakizawa, M.Nishi, and H.Takeshima.
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Journal Title
science signaling
Volume: 9
Issue: 428
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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