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Development of a method to isolate molecules in cellular structures where fluorescent proteins localize

Research Project

Project/Area Number 15K14500
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionNational Institute of Information and Communications Technology

Principal Investigator

Matsuda Atsushi  国立研究開発法人情報通信研究機構, 未来ICT研究所フロンティア創造総合研究室, 主任研究員 (20585723)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsバイオイメージング / 低温工学
Outline of Final Research Achievements

This study is intended to develop a method to isolate frozen cellular micro-compartments relying on fluorescence of localized proteins. We examined methods for crushing ice into small particles, which is suitable for sorting by fluorescence, using bullet fracturing and beads milling. Also, we have designed and built a sorter microscope to sort ice particles containing cellular micro-compartments using fluorescence under low temperature. Simultaneously we have developed a system that prevents solidification of water vapor on the optical components. Using this microscope, we were able to observe ice particles made by bullet fracturing under low temperature, and found that these ice particles are below 10 micrometers in diameter.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (33 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Acknowledgement Compliant: 6 results,  Open Access: 8 results) Presentation (12 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results) Book (1 results) Remarks (7 results)

  • [Int'l Joint Research] オックスフォード大学(英国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Quantitative 3D structured illumination microscopy of nuclear structures2017

    • Author(s)
      Felix Kraus, Ezequiel Miron, Justin Demmerle, Tsotne Chitiashvili, Alexei Busco, Quentin Alle, Atsushi Matsuda, Heinrich Leonhardt, Lothar Schermelleh & Yolanda Markaki
    • Journal Title

      Nature Protocols

      Volume: 12 Issue: 5 Pages: 1011-1028

    • DOI

      10.1038/nprot.2017.020

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Strategic and practical guidelines for successful structured illumination microscopy2017

    • Author(s)
      Justin Demmerle, Cassandravictoria Innocent, Alison J North, Graeme Ball, Marcel Muller, Ezequiel Miron, Atsushi Matsuda, Ian M Dobbie, Yolanda Markaki & Lothar Schermelleh
    • Journal Title

      Nature Protocols

      Volume: 12 Issue: 5 Pages: 988-1010

    • DOI

      10.1038/nprot.2017.019

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Spatial organization of the Schizosaccharomyces pombe genome within the nucleus.2017

    • Author(s)
      Matsuda A, Asakawa H, Haraguchi T, Hiraoka Y.
    • Journal Title

      Yeast.

      Volume: 34 Issue: 2 Pages: 55-66

    • DOI

      10.1002/yea.3217

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Borna Disease Virus Assembles Porous Cage-like Viral Factories in the Nucleus.2016

    • Author(s)
      Hirai Y, Hirano Y, Matsuda A, Hiraoka Y, Honda T, Tomonaga K.
    • Journal Title

      The Journal of biological chemistry.

      Volume: 291 Issue: 50 Pages: 25789-25798

    • DOI

      10.1074/jbc.m116.746396

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meiotic cohesin subunits RAD21L and REC8 are positioned at distinct regions between lateral elements and transverse filaments in the synaptonemal complex of mouse spermatocytes2016

    • Author(s)
      Rong M, Matsuda A, Hiraoka Y, Lee J
    • Journal Title

      J Reprod Dev

      Volume: 62 Issue: 6 Pages: 623-630

    • DOI

      10.1262/jrd.2016-127

    • NAID

      130005251186

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 実践編3 原理・応用編3 構造化照明顕微鏡法SIM ‐縞照明のつくるモアレが可能にする超解像観察2016

    • Author(s)
      松田厚志、平野泰弘、平岡泰
    • Journal Title

      実験医学別冊・初めてでもできる! 超解像イメージング(羊土社)

      Volume: 別冊 Pages: 219-226

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 実践編3 3D-SIMによる細胞内構造の超解像イメージング---アーティファクトの少ないSIM画像の取得2016

    • Author(s)
      平野泰弘、松田厚志、平岡泰
    • Journal Title

      実験医学別冊・初めてでもできる! 超解像イメージング(羊土社)

      Volume: 別冊 Pages: 146-155

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.2016

    • Author(s)
      Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J.
    • Journal Title

      Nature Commun.

      Volume: 7 Issue: 1 Pages: 10393-10393

    • DOI

      10.1038/ncomms10393

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast.2015

    • Author(s)
      Matsuda A, Chikashige Y, Ding DQ, Ohtsuki C, Mori C, Asakawa H, Kimura H, Haraguchi T, Hiraoka Y.
    • Journal Title

      Nature Communication

      Volume: 6 Issue: 1 Pages: 1-12

    • DOI

      10.1038/ncomms8753

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins.2015

    • Author(s)
      Poonperm R, Takata H, Hamano T, Matsuda A, Uchiyama S, Hiraoka Y, Fukui K
    • Journal Title

      Sci. Rep.

      Volume: 5 Issue: 1 Pages: 11916-11916

    • DOI

      10.1038/srep11916

    • NAID

      120006995010

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recent advancements in structured-illumination microscopy toward live-cell imaging.2015

    • Author(s)
      Hirano Y, Matsuda A, Hiraoka Y
    • Journal Title

      Microscopy (Oxf)

      Volume: 64 Issue: 4 Pages: 237-249

    • DOI

      10.1093/jmicro/dfv034

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Meiotic cohesion-based chromosome structure is essential for homologous chromosome pairing in Schizosaccharomyces pombe2015

    • Author(s)
      Ding DQ, Matsuda A, Okamasa K, Nagahama Y, Haraguchi T, Hiraoka Y
    • Journal Title

      Chromosoma

      Volume: special issue Issue: 2 Pages: 1-10

    • DOI

      10.1007/s00412-015-0551-8

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] THREE-DIMENSIONAL CHROMATIC CORRECTION FOR FLUORESCENCE MICROSCOPY ENABLING PRECISE DISTANCE MEASUREMENTS IN THE NUCLEAR PORE COMPLEX2017

    • Author(s)
      Atsushi Matsuda, Haruhiko Asakawa, Yasuhiro Hirano, Lothar Schermelleh, Tokuko Haraguchi, and Yasushi Hiraoka
    • Organizer
      Focus on Microscopy 2017
    • Place of Presentation
      Bordeaux, France
    • Year and Date
      2017-04-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Super-resolution imaging and precise distance measurements in live fission yeast cells The 14th International Congress on Yeast2016

    • Author(s)
      Atsushi Matsuda, Tokuko Haraguchi, Yasushi Hiraoka
    • Organizer
      The 14th International Congress on Yeasts
    • Place of Presentation
      淡路島夢舞台国際会議場、兵庫県淡路市
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Super-Resolution and Chromatic Correction Above the Cover Slip2016

    • Author(s)
      Atsushi Matsuda, Yasuhiro Hirano, Tokuko Haraguchi and Yasushi Hiraoka
    • Organizer
      Biomedical Imaging and Sensing Conference 2016
    • Place of Presentation
      パシフィコ横浜, 神奈川県横浜市
    • Year and Date
      2016-05-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Super-Resolution and Chromatic Correction Above the Cover Slip2016

    • Author(s)
      Matsuda A, Hirano Y, Haraguchi T, Hiraoka Y
    • Organizer
      BISC2016
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2016-05-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 回折限界を超えた蛍光顕微鏡で細胞核を見る2016

    • Author(s)
      松田厚志
    • Organizer
      第10回NIBBバイオイメージングフォーラム「新時代のバイオイメージングの開拓」
    • Place of Presentation
      基礎生物学研究所、愛知県岡崎市
    • Year and Date
      2016-02-16
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 染色体スキャフォールドはスキャフォールドタンパク質の2本鎖構造をもつ2015

    • Author(s)
      Poonperm R, 高田 英昭、濱野 徹、松田 厚志、内山 進、平岡 泰、福井 希一
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場、兵庫県神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 回折限界を超えた蛍光顕微鏡とクロマチン高次構造2015

    • Author(s)
      松田厚志
    • Organizer
      本能構造の発現メカニズムに関する総合科学研究推進拠点第8回研究セミナー
    • Place of Presentation
      広島大学、広島県東広島市
    • Year and Date
      2015-10-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast2015

    • Author(s)
      Matsuda A, Hirano Y, Haraguchi T, Hiraoka Y
    • Organizer
      International Symposium on Chromatin Structure, Dynamics, and Function
    • Place of Presentation
      Yumebutai International Conference Center、兵庫県淡路市
    • Year and Date
      2015-08-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Shugoshin forms a specialized chromatin domain at subtelomeres, repressing transcription and replication2015

    • Author(s)
      Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J
    • Organizer
      Pombe2015 8th International Fission Yeast Meeting
    • Place of Presentation
      生田神社会館、兵庫県神戸市
    • Year and Date
      2015-06-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Super-resolution microscopy reveals highly condensed chromatin adjacent to subtelomeric, decondensed silent chromatin2015

    • Author(s)
      Matsuda A, Hirano Y, Haraguchi T, Hiraoka Y
    • Organizer
      Pombe2015 8th International Fission Yeast Meeting
    • Place of Presentation
      生田神社会館、兵庫県神戸市
    • Year and Date
      2015-06-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 3D-SIM Analysis of Meiotic Chromosome Structure in Live Cells of S.pombe2015

    • Author(s)
      Ding DQ, Matsuda A, Haraguchi T, Hiraoka Y
    • Organizer
      Pombe2015 8th International Fission Yeast Meeting
    • Place of Presentation
      生田神社会館、兵庫県神戸市
    • Year and Date
      2015-06-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 分解能の壁を越えて見る染色体高次構造2015

    • Author(s)
      松田厚志
    • Organizer
      顕微鏡学会第71回学術講演会
    • Place of Presentation
      国立京都国際会館、京都府京都市
    • Year and Date
      2015-05-13
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 新・生細胞蛍光イメージング2015

    • Author(s)
      松田厚志
    • Total Pages
      340
    • Publisher
      共立出版
    • Related Report
      2015 Research-status Report
  • [Remarks] 情報通信研究機構・未来ICT研究所・生物情報グループ

    • URL

      http://www2.nict.go.jp/frontier/seibutsu/CellMagic/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学細胞核ダイナミクス研究室

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学生命機能研究科最新研究成果

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/events/achievement/matsuda-hiraoka-20150724/

    • Related Report
      2015 Research-status Report
  • [Remarks] ニュース

    • URL

      https://www.sciencedaily.com/releases/2016/03/160328085139.htm

    • Related Report
      2015 Research-status Report
  • [Remarks] ニュース

    • URL

      http://phys.org/news/2016-03-function-chromosomal-terminus-vital-human.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 情報通信研究機構生物情報グループ

    • URL

      http://www2.nict.go.jp/advanced_ict/bio/w131103/CellMagic/

    • Related Report
      2015 Research-status Report
  • [Remarks] 大阪大学平岡研究室

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2022-02-16  

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