| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
This study is intended to develop a method to isolate frozen cellular micro-compartments relying on fluorescence of localized proteins. We examined methods for crushing ice into small particles, which is suitable for sorting by fluorescence, using bullet fracturing and beads milling. Also, we have designed and built a sorter microscope to sort ice particles containing cellular micro-compartments using fluorescence under low temperature. Simultaneously we have developed a system that prevents solidification of water vapor on the optical components. Using this microscope, we were able to observe ice particles made by bullet fracturing under low temperature, and found that these ice particles are below 10 micrometers in diameter.
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