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Development of a new freeze replica method to observe the hydrophobic portion of membrane lipids

Research Project

Project/Area Number 15K14506
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionNagoya University

Principal Investigator

Fujimoto Toyoshi  名古屋大学, 医学系研究科, 教授 (50115929)

Co-Investigator(Kenkyū-buntansha) 辻 琢磨  名古屋大学, 医学系研究科, 助教 (40725628)
Research Collaborator MAKOTO ARITA  独立行政法人理化学研究所, 総合生命医科学研究センター, チームリーダー (80292952)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords膜脂質 / 電子顕微鏡
Outline of Final Research Achievements

Phospholipids constitute the basic framework of biological membranes and they are known to contain highly variable acyl chains in their hydrophobic tail portion. However, it is hardly known how those acyl chains distribute in the cellular membrane. In this project, we developed a method to define the nano-scale distribution of a specific kind of acyl chain by utilizing alkyne-labeled fatty acids and quick-freezing freeze-fracture electron microscopy. By performing click reaction on the freeze-fracture replica, labeling for the acyl chain was observed in cellular membranes, and moreover, it was shown to form clusteres in a limited region of the membrane.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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