Development of live-cell assay system for monitoring phospholipid transport
| Project/Area Number |
15K14507
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リン脂質 / オルガネラ / 人工酵素 |
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| Outline of Final Research Achievements |
It has been largely unknown how phospholipid, major constituents of cellular membranes, travel within cells due to lack of reliable assay system to analyze phospholipid transport. To overcome this shortcoming, we tried to develop a novel assay system for directly measuring phospholipid transport in cells. By expressing PssA, which is the E. coli PS (phosphatidylserine) synthetic enzyme in a number of cellular compartments within yeast cells, we succeeded to observe PS transport from various organelles to mitochondria.
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Report
(3 results)
Research Products
(31 results)
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[Journal Article] Crystal Structure of Human General Transcription Factor TFIIE at Atomic Resolution2016
Author(s)
Miwa, K., Kojima, R., Obita, T., Ohkuma, Y., Tamura, Y., and Mizuguchi, M.
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Journal Title
JOURNAL OF MOLECULAR BIOLOGY
Volume: 428
Issue: 21
Pages: 4258-4266
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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