| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
It has been largely unknown how phospholipid, major constituents of cellular membranes, travel within cells due to lack of reliable assay system to analyze phospholipid transport. To overcome this shortcoming, we tried to develop a novel assay system for directly measuring phospholipid transport in cells. By expressing PssA, which is the E. coli PS (phosphatidylserine) synthetic enzyme in a number of cellular compartments within yeast cells, we succeeded to observe PS transport from various organelles to mitochondria.
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