| Project/Area Number |
15K14508
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | LRRK1 / 中心小体 / ライセンシングシグナル / 中心体 / 母娘中心小体 / セパレース / セパレースセンサー |
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| Outline of Final Research Achievements |
The centrosome consists of a pair of mother-daughter centrioles, which are replicated only once per cell cycle. At this time, the disengagement of mother-daughter centrioles acts as a licensing signal for centriole duplication. Dissociation of the mother-daughter centrioles occurs by the cleavage of cohesin by the protease separator. When a separator sensor with a chromophore on both sides of the cohesin cleavage sequence is used, the activity of the separase can be observed with a change in fluorescence. In this study, we used this system to identify genes important for licensing signals and analyze their molecular mechanisms. As a result, it was revealed that the ROCO family kinase LRRK1 controls the disengagement of the mother-daughter centrioles in a kinase activity-dependent manner.
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