Molecular mechanisms underlying novel oxidative stress response and cellular homeostasis by coordination of peroxisomes and mitochondria
Project/Area Number |
15K14511
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Cell biology
|
Research Institution | Kyushu University |
Principal Investigator |
Fujiki Yukio 九州大学, 生体防御医学研究所, 特任教授 (70261237)
|
Co-Investigator(Renkei-kenkyūsha) |
OKUMOTO KANJI 九州大学, 大学院理学研究院, 助教 (20363319)
HONSHO MASANORI 九州大学, 生体防御医学研究所, 特任准教授 (90372747)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | ペルオキシソーム / カタラーゼ / BAK / VDAC2 / 酸化ストレス / 細胞死抑制 / アポトーシス / ミトコンドリア / オルガネラ形成・形態 / レドックスバランス |
Outline of Final Research Achievements |
We found that VDAC2 encoding a mitochondrial porin is a causal gene of a peroxisome-deficient CHO mutant, ZP114 showing the impaired import of peroxisome matrix proteins such as catalase. Loss of VDAC2 leads to localization shift of a proapoptotic factor BAK from mitochondria to peroxisomes, resulting in permeabilization of peroxisomal membrane in a manner similar to mitochondrial outer membrane. A part of BAK potentially localizes to peroxisomes and regulates peroxisome integrity and the release of catalase from peroxisomes to the cytosol in normal cells, which can counteracts with commitment of apoptosis by various oxidative stresses including reactive oxygen species.
|
Report
(3 results)
Research Products
(59 results)
-
-
-
[Journal Article] The VDAC2-BAK axis regulates peroxisomal membrane permeability.2017
Author(s)
Hosoi, K., Miyata, N., Mukai, S., Furuki, S., Okumoto, K., Cheng, E. H., and *Fujiki, Y.
-
Journal Title
J. Cell Biol.
Volume: 216
Issue: 3
Pages: 709-722
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] Defining dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae.2017
Author(s)
Imoto, Y., Abe, Y., Okumoto, K., Honsho, M., Kuroiwa, H., Kuroiwa, T., and *Fujiki, Y.
-
Journal Title
J. Cell Sci.
Volume: 130
Pages: 853-867
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Localization of protein kinase NDR2 to peroxisomes and its role in ciliogenesis.2017
Author(s)
Abe, S., Nagai, T., Masukawa, M., Okumoto, K., Homma, Y., Fujiki, Y., and Mizuno, K.
-
Journal Title
J. Biol. Chem.
Volume: 292
Issue: 10
Pages: 4089-4098
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
[Journal Article] Reduction of ether-type glycerophospholipids, plasmalogens, by NF-κB signal leading to microglial activation2017
Author(s)
Hossain, Md. S., Abe, Y., Ali, F., Youssef, M., Honsho, M., Fujiki, Y., and *Katafuchi, T.
-
Journal Title
J. Neurosci.
Volume: 37
Issue: 15
Pages: 4074-4092
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] Experimental systems to study yeast pexophagy. In: Schrader, M. (ed.) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed.: Walker, J.M.)2017
Author(s)
Yamashita, S., Oku, M., Sakai, Y., and Fujiki, Y.
Total Pages
7
Publisher
Springer, Humana Press, New York, USA
Related Report
-
[Book] In vitro PMP import analysis using cell-free synthesized PMP and isolated peroxisomes. In: Schrader, M. (ed.) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed.: Walker, J.M.)2017
Author(s)
Liu,Y., Honsho, M., and Fujiki, Y.
Total Pages
6
Publisher
Springer, Humana Press, New York, USA
Related Report
-
-
[Book] Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system. In: Schrader, M. (ed.) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed.: Walker, J.M.)2017
Author(s)
Okumoto, K., Honsho, M., Liu, Y., and Fujiki, Y.
Total Pages
7
Publisher
Springer, Humana Press, New York, USA
Related Report
-
-
[Book] Blue-Native PAGE: Applications to study peroxisome biogenesis. In: Schrader, M. (ed.) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed.: Walker, J.M.)2017
Author(s)
Okumoto, K., Tamura, S., and Fujiki, Y.
Total Pages
9
Publisher
Springer, Humana Press, New York, USA
Related Report
-
-
-
-
-
-