Project/Area Number |
15K14514
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Cell biology
|
Research Institution | National Institute of Genetics |
Principal Investigator |
Sawa Hitoshi 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (80222024)
|
Project Period (FY) |
2015-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
Keywords | エピジェネティックス / C. elegans / イメージング / Wnt / 非対称細胞分裂 / ヒストン修飾 / 線虫 |
Outline of Final Research Achievements |
Using C. elegans, we tried to visualize chromatin status of a specific gene (egl-18). We established a reporter array containing hundreds copies of the gene promoter and the lacO sequence, and measured changes of nuclear positions of the array during cell differentiation using lacI::GFP, enabling us to visualize dynamics of a single gene locus during development. In addition, by immunostaining using anti-modified histone antibodies, we succeeded to visualize dynamic changes of histone marks on this gene during development.
|