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Elucidating the force-based mechanisms regulating nuclear structure and function

Research Project

Project/Area Number 15K14515
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionNational Institute of Genetics

Principal Investigator

Shimamoto Yuta  国立遺伝学研究所, 新分野創造センター, 准教授 (80409656)

Research Collaborator MAESHIMA Kazuhiro  国立遺伝学研究所, 生体高分子研究室, 教授
TAMURA Sachiko  国立遺伝学研究所, 生体高分子研究室, テクニシャン
MASUMOTO Hiroshi  長崎大学, 医学部, 講師
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞核 / 機械計測 / クロマチン / マイクロマニピュレーション / 力学特性 / ヒストン / DNA / 生物物理学 / 力計測 / 物性 / 力学情報変換
Outline of Final Research Achievements

This study focused on elucidating the physical and molecular basis underlying nuclear mechanical response, with an ultimate goal to understand cell’s mechanotransduction mechanisms. It had long been recognized that mechanical force acting on a cell can transmit across the cytoplasm and alter the structure and function of the nucleus. However, it was largely unknown how the nucleus resists and responds to the force, due primarily to the lack of a suitable assay. To address this, we developed a microneedle-based micromanipulation setup and quantitatively examined the mechanical response of human cell nuclei, such as those of HeLa cells. Combining biochemical perturbation assays, our measurements revealed that chromatin can respond to stretching force that acts on the nucleus, and that the chromatin’s response to force is significantly suppressed upon digestion of DNA or acetylation of histones.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Nucleosome-nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity2017

    • Author(s)
      Shimamoto Y, Tamura S, Masumoto H, Maeshima K
    • Journal Title

      Molecular Biology of the Cell

      Volume: in press Issue: 11 Pages: 1580-1589

    • DOI

      10.1091/mbc.e16-11-0783

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] クロマチン脱凝集が細胞核の力学特性に与える効果2017

    • Author(s)
      島本勇太
    • Organizer
      日本機械学会第29回バイオエンジニアリング講演会
    • Place of Presentation
      ういんくあいち、名古屋
    • Year and Date
      2017-01-18
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Nucleosome-nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity2016

    • Author(s)
      Yuta Shimamoto
    • Organizer
      The American Society for Cell Biology 2016 Annual Meeting
    • Place of Presentation
      Moscone Center, San Fransisco, CA
    • Year and Date
      2016-12-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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