Characterization of mechanisms of mRNA localization in the ascidian eggs with the novel genetic method to knockdown maternal genes
| Project/Area Number |
15K14520
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ホヤ / mRNA / 母性因子 / piRNA / 局在 / 発生・分化 / 生殖細胞 / RNA局在 |
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| Outline of Final Research Achievements |
A group of mRNA is localized at the specific region in eggs of ascidians. Mechanisms of the mRNA localization have not been understood. We have recently developed the novel genetic method MASK that knocks down genes in the eggs in the ascidian Ciona. The mechanisms how maternal genes are knocked down by MASK was unknown. In this research, we showed the mechanisms of MASK. In the MASK transgenic lines, piRNA-like small RNAs are produced from the MASK vector in the transcription-dependent manner. The piRNA-like small RNAs induce degradation of mRNA of targeted maternal gene. Because piRNAs are produced by the ping-pong mechanism, the addition of such an element in the MASK vector will increase the efficiency to knockdown target genes by MASK.
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Report
(4 results)
Research Products
(6 results)
