Identification of blastocoel fluid components and its function in early development
Project/Area Number |
15K14521
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | The University of Tokyo |
Principal Investigator |
KONDO Mariko 東京大学, 大学院理学系研究科(理学部), 准教授 (70372414)
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Co-Investigator(Kenkyū-buntansha) |
高橋 秀治 広島大学, 両生類研究センター, 特任准教授 (90447318)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | アフリカツメガエル / 胞胚腔 / 初期発生 / プロテオーム解析 / ツメガエル / プロテオミクス |
Outline of Final Research Achievements |
The role of the blastocoel in early embryos are unknown. In this study, we hypothesized that it functions as the space which is used for signaling between tissues or for cell proliferation, and tried to verify this using proteomics of the blastocoel fluid. We identified proteins that are involved in iron ion transport or homeostasis, proteinases, etc. However, we could not detect ligand proteins involved in cell signaling, and our hypothesis was not supported. The results suggested that the blastocoel may be utilized as a pool to digest unnecessary proteins during early embryogenesis.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Conservatism and variability of gene expression profiles among homeologous transcription factors in Xenopus laevis.2017
Author(s)
Watanabe, M., Yasuoka, Y., Mawaribuchi, S., Kuretani, A., Ito, M., Kondo, M., Ochi, H., Ogino, H., Fukui, A., Taira, M. and Kinoshita, T.
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Journal Title
Dev. Biol.
Volume: 426
Issue: 2
Pages: 301-324
DOI
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Peer Reviewed / Open Access
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[Journal Article] Sex chromosome differentiation and the W- and Z-specific loci in Xenopus laevis.2016
Author(s)
Mawaribuchi, S., Takahashi, S., Wada, M., Uno, Y., Matsuda, Y., Kondo, M., Fukui, A., Takamatsu, N., Taira, M., and Ito, M.
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Journal Title
Dev Biol
Volume: -
Issue: 2
Pages: 393-400
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Xenopus laevis全ゲノム解析:アフリカツメガエルnodal5とnodal6遺伝子クラスターについての解析2015
Author(s)
高橋 秀治, 豊田 敦, 宇野 好宣, 黒木 陽子, 彦坂 暁, 原本 悦和, 田中 利明, 西城 智仁, 野口 英樹, 松田 洋一, 近藤 真理子, 藤山 秋佐夫, 上野 直人, 平良 眞規, 浅島 誠
Organizer
第38回 日本分子生物学会年会
Place of Presentation
神戸ポートアイランド(兵庫県神戸市)
Year and Date
2015-12-01
Related Report
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