Establishment of recombinant rabies virus-mediated retrograde trancing of neural circuits in zebrafish
Project/Area Number |
15K14523
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | Nagoya University |
Principal Investigator |
HIBI MASAHIKO 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
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Co-Investigator(Renkei-kenkyūsha) |
OSAKADA FUMITAKA 名古屋大学, 創薬科学研究科, 准教授 (60455334)
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Research Collaborator |
DOHAKU RYUJI 名古屋大学, 理学研究科
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 逆行性トレーシング / 狂犬病ウイルス / 神経回路 / 神経解剖学 / 小脳 / 苔状線維 / 登上線維 / ゼブラフィッシュ / 逆光性トレーシング |
Outline of Final Research Achievements |
Rabies virus (RV) infects neurons through its glycoprotein (G). Recombinant RV that contains the GFP gene instead of the G gene and is pseudotyped with the retroviral envelope protein (EnvA) was developed for neural circuit tracing. The pseudotyped RV can infect neurons that express TVA (the EnvA receptor)-mCherry. If the G is provided in the same cells, the RV particles are generated and transmitted mono-synaptically to input neurons. We generated transgenic lines that express TVA-mCherry and G in Purkinje cells (PCs) or granule cells (GCs). When PCs were infected with the pseudotyped RV, GFP was detected in GCs and neurons in the inferior olive nuclei. When GCs were infected with the virus, GFP was detected in neurons in various brain regions that receive inputs from the visual, vestibular, lateral line sensory systems, or telencephalon. Our findings validated the RV-mediated retrograde tracing in zebrafish and identified input neurons for GCs in zebrafish.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] REP1 inhibits FOXO3-mediated apoptosis to promote cancer cell survival.2017
Author(s)
Song KH, Woo SR, Chung JY, Lee HJ, Oh SJ, Hong SO, Shim J, Kim YN, Rho SB, Hong SM, Cho H, Hibi M, Bae DJ, Kim SY, Kim MG, Kim TW, Bae YK.
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Journal Title
Cell Death & Disease
Volume: 8
Issue: 1
Pages: e2536-e2536
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Osteocrin, a secretory peptide from heart, contributes to cranial osteogenesis in zebrafish2016
Author(s)
Chiba A, Watanabe-Takano H, Terai K, Fukui H, Miyazaki T, Uemura M, Hashimoto H, Hibi M, Fukuhara S, Mochizuki N
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Journal Title
Development
Volume: 144
Pages: 334-344
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] ype IV collagen controls the axogenesis of cereberr granule cells by regulating basement membrane integrity in zebrafish.2015
Author(s)
Takeuchi, M., Yamaguchi, S., Yonemura, S., Kakiguchi, K., Sato, Y., Higashiyama, T., Shimizu, T., and, Hibi, M.
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Journal Title
PLoS genet.
Volume: 11
Issue: 10
Pages: e1005587-e1005587
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination.2015
Author(s)
Kim, J.D., Park, K.E., Ishida, J., Kako, K., Hamada, J., Kani, S., Takeuchi, M., Namiki, K., Fukui, H., Fukuhara, S., Hibi, M,. Kobayashi, M., Kanaho, Y., Kasuya, Y., Mochizuki, N., Fukamizu, A.
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Journal Title
Sci. Adv.
Volume: 1
Issue: 11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Evolution and development of cerebellar neural circuitry in teleosts.2015
Author(s)
Hibi M, Takeuchi M, Yamaguchi S, Matsuda K, Hayashi T, Hara Y, Hayashi T, Sakakibara Y, Yoshida M, Kuraku S, Shimizu T.
Organizer
第48回日本発生生物学会大会
Place of Presentation
つくば
Year and Date
2015-06-02
Related Report
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