| Project/Area Number |
15K14529
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kyushu University |
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Principal Investigator |
Chikara Meno 九州大学, 医学研究院, 教授 (20311764)
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| Co-Investigator(Renkei-kenkyūsha) |
OKI Shinya 九州大学, 医学研究院, 助教 (90452713)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 転写因子 / ゲノムDNA / PLA法 / ゲノム / 発現制御 / タンパク質-DNA相互作用 |
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| Outline of Final Research Achievements |
Although chromatin immunoprecipitation is a useful assay that probes protein-genomic DNA interactions in nuclei, it requires a lot of cells and the purification of specific cells by FACS when tissues are examined. We attempted to develop a general method that detects binding of specific proteins to genomic DNA on tissue sections. We developed the method by examining binding of Pou5f1 to Pou5f1 enhancer in P19 cells as a model case, and obtained results that suggest their interactions. In order to make the method reliable, however, further investigation is required.
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