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Development of a technology that can visualize interactions between genomic DNA and proteins in nuclei in situ

Research Project

Project/Area Number 15K14529
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKyushu University

Principal Investigator

Chikara Meno  九州大学, 医学研究院, 教授 (20311764)

Co-Investigator(Renkei-kenkyūsha) OKI Shinya  九州大学, 医学研究院, 助教 (90452713)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords転写因子 / ゲノムDNA / PLA法 / ゲノム / 発現制御 / タンパク質-DNA相互作用
Outline of Final Research Achievements

Although chromatin immunoprecipitation is a useful assay that probes protein-genomic DNA interactions in nuclei, it requires a lot of cells and the purification of specific cells by FACS when tissues are examined. We attempted to develop a general method that detects binding of specific proteins to genomic DNA on tissue sections. We developed the method by examining binding of Pou5f1 to Pou5f1 enhancer in P19 cells as a model case, and obtained results that suggest their interactions. In order to make the method reliable, however, further investigation is required.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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