Localized Wnt protein and morphogenesis.

• Project/Area Number: 15K14532
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Mii Yusuke 基礎生物学研究所, 分子発生学研究部門, 助教 (70634129)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Wnt / 平面細胞極性 / Xenopus / ヘパラン硫酸 / PCP / アフリカツメガエル / noncanonical Wnt / 細胞骨格

Outline of Final Research Achievements

How Wnt proteins regulate morphogenesis and cell polarity is poorly understood. From visualization of Wnt11 in early Xenopus embryos, following possibilities are suggested. 1. Wnt11 shows different specificity for heparan sulfate from that of Wnt8. 2. Cell edges with Wnt11 accumulation tend to shrink. 3. For the regulation of planar cell polarity (PCP), Wnt11 appears to regulate amounts of core PCP components in a localized manner.

Research Products

• [Journal Article] Roles of two types of heparan sulfate clusters in Wnt distribution and signaling in Xenopus (2018)
  • Author(s): Yusuke Mii, Takayoshi Yamamoto, Ritsuko Takada, Shuji Mizumoto, Makoto Matsuyama, Shuhei Yamada, Shinji Takada, and Masanori Taira
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1973-1973
  • DOI: 10.1038/s41467-017-02076-0
  • Peer Reviewed / Open Access
• [Journal Article] Ripply3 is required for the maintenance of epithelial sheets in the morphogenesis of pharyngeal pouches (2018)
  • Author(s): Tsuchiya, Y., Mii, Y., Okada, K., Furuse, M., Okubo, T., Takada, S.
  • Journal Title: Dev. Growth Diff. Volume: 60 Issue: 2 Pages: 87-96
  • DOI: 10.1111/dgd.12425
  • Peer Reviewed
• [Journal Article] Differences in the secretion and transport of Wnt proteins (2017)
  • Author(s): Takada S, Fujimori S, Shinozuka T, Takada R, Mii Y.
  • Journal Title: J Biochem. Volume: 161 Issue: 1 Pages: 1-7
  • DOI: 10.1093/jb/mvw071
  • Peer Reviewed
• [Presentation] Local and mutual regulations between Wnt and planar cell polarity propagate global coordination of cell polarity through the tissue. (2018)
  • Author(s): Yusuke Mii, Ritsuko Takada, Makoto Matsuyama, Shinji Takada
  • Organizer: 日本発生生物学会第51回大会
  • Invited
• [Presentation] Spatial regulation of Wnt proteins and planar cell polarity in Xenopus early embryogenesis. (2017)
  • Author(s): Yusuke Mii, Ritsuko Takada, Makoto Matsuyama, Shinji Takada
  • Organizer: 日本発生生物学会第50回大会
• [Presentation] Differential modification defines discrete nano-scale clusters of heparan sulfate and coordinates gradient formation and signal reception of Wnt in Xenopus embryo. (2016)
  • Author(s): Yusuke Mii, Takayoshi Yamamoto, Ritsuko Takada, Masanori Taira, Shinji Takada
  • Organizer: CDB symposium
  • Place of Presentation: 多細胞システム形成研究センター（兵庫県神戸市）
  • Year and Date: 2016-03-28
  • Int'l Joint Research
• [Presentation] ヘパラン硫酸ナノ構造による Wnt の局在化と細胞極性 (2015)
  • Author(s): 三井 優輔
  • Organizer: 次世代両生類研究会 第一回会合
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2015-08-24
  • Invited
• [Presentation] Spatial regulation of Wnt proteins and convergent extension movements during Xenopus early embryogenesis. (2015)
  • Author(s): Yusuke Mii, Takayoshi Yamamoto, Ritsuko Takada, Masanori Taira, Shinji Takada
  • Organizer: 日本発生生物学会第48回大会
  • Place of Presentation: つくば国際会議場、（茨城県つくば市）
  • Year and Date: 2015-06-02
• [Remarks] 分子発生学研究部門（高田研究室）
  • URL: http://www.nibb.ac.jp/cib2/