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Study of cell morphology and intercellular-signaling simulation using the artificial control systems in leaf epidermal-cell morphogenesis

Research Project

Project/Area Number 15K14536
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

HASEZAWA Seiichiro  東京大学, 大学院新領域創成科学研究科, 教授 (40172902)

Co-Investigator(Renkei-kenkyūsha) Miura Takashi  九州大学, 医学(系)研究科(研究院), 教授 (10324617)
Kutsuna Natsumaro  東京大学, 大学院新領域創成科学研究科, 特任准教授 (70578559)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords成長生理 / 細胞形態形成 / 植物ホルモン / シミュレーション / 数理モデル
Outline of Final Research Achievements

We analyzed morphogenetic process of Arabidopsis thaliana leaf epidermal cells with complex morphology, and visualized cell shape and cell connection mode at the tissue level. In particular, when cellulase (digesting cellulose in the cell wall), was treated on leaves of plants, the leaf epidermal cells change from a complicated intricate configuration like a jigsaw puzzle to a straight extension. We focused on microtubule-associated protein called RIC1, which was known to play an important role in the formation of jigsaw puzzle type of leaf cells, and analyzed the role of RIC1 in the cellulase response. As a result, we found that no significant change was observed in the cell shape of the jigsaw puzzle even when the cellulase was treated with the mutant lacking RIC1.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (8 results)

  • [Journal Article] Exogenous cellulase switches cell interdigitation to cell elongation in a RIC1-dependent manner in Arabidopsis thaliana cotyledon pavement cells.2017

    • Author(s)
      Higaki T, Takigawa-Imamura H, Akita K, Kutsuna N, Kobayashi R, Hasezawa S, Miura T
    • Journal Title

      Plant Cell Physiol

      Volume: 58 Pages: 106-119

    • DOI

      10.1093/pcp/pcw183

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals.2017

    • Author(s)
      Takahashi, S., Monda, K., Higaki, T., Negi, J., Hashimoto-Sugimoto, M., Hasezawa, S. and Iba, K.
    • Journal Title

      Front. Plant Sci.

      Volume: 8 Pages: 677-677

    • DOI

      10.3389/fpls.2017.00677

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Differential responses to high- and low-dose ultraviolet-B stress in tobacco Bright Yellow-2 cells2015

    • Author(s)
      Takahashi S, Kojo K.H, Kutsuna N, Endo M, Toki S, Isoda H, Hasezawa S
    • Journal Title

      Frontiers in Plant Science

      Volume: 6 Pages: 254-254

    • DOI

      10.3389/fpls.2015.00254

    • NAID

      120007136348

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A high molecular weight zinc transporter MTP12 forms a functional heteromeric complex with MTP5 in the Golgi in Arabidopsis thaliana.2015

    • Author(s)
      Fujiwara T, Kawachi M, Sato Y, Mori H, Kutsuna N, Hasezawa S, Maeshima M
    • Journal Title

      FEBS Journal

      Volume: 282 Pages: 1965-1979

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantitative analysis of microtubule orientation in interdigitated leaf pavement cells.2015

    • Author(s)
      Kae Akita, Takumi Higaki, Natsumaro Kutsuna, Seiichiro Hasezawa
    • Journal Title

      Plant Signaling & Behavior

      Volume: 印刷中 Issue: 5 Pages: e1024396-e1024396

    • DOI

      10.1080/15592324.2015.1024396

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 子葉表皮細胞壁の湾曲における微小管結合タンパク質RIC1の役割:細胞形態計測と力学モデルによる解析2017

    • Author(s)
      桧垣匠,今村寿子,秋田佳恵,朽名夏麿,三浦岳,馳澤盛一郎
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高CO2処理が孔辺細胞の分布および表皮細胞の形態に及ぼす影響の解析2017

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] スクロース水浸処理により誘導される気孔クラスターの細胞形態学的解析2016

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] PI4Kインヒビター(Phenylarsine oxide)による気孔開閉阻害に対して低感受性を示すシロイヌナズナ変異体の単離2016

    • Author(s)
      高橋將,門田慧奈,桧垣匠,馳澤盛一郎,橋本(杉本)美海,祢宜淳太郎,射場厚
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物細胞におけるゴルジ体形成維持機構の解析2016

    • Author(s)
      伊藤容子,植村知博,湖城恵,馳澤盛一郎,上田貴志,中野明彦
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学上田キャンパス(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] シロイヌナズナ葉表皮細胞における細胞壁湾曲機構の解析2015

    • Author(s)
      桧垣匠,今村寿子,秋田佳恵,朽名夏麿,馳澤盛一郎,三浦岳
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] 顕微鏡画像の網羅的解析のためのクラスタリング法の開発2015

    • Author(s)
      朽名夏麿,馳澤盛一郎
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] BY-2細胞における表層微小管配向再形成過程の画像定量解析2015

    • Author(s)
      佐野行巳,朽名夏麿,馳澤盛一郎
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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