| Project/Area Number |
15K14537
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TERASHIMA Ichiro 東京大学, 大学院理学系研究科(理学部), 教授 (40211388)
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| Research Collaborator |
FUJITA Takashi 東京大学, 理学系研究科
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アポプラスト / 気孔 / 葉肉シグナル / 光合成 / 青色光 / CO2 / シグナル伝達 |
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| Outline of Final Research Achievements |
Transplanting of the isolated epidermis onto the mesophyll is a potent methodology for examination of roles of mesophyll-derived signals in controlling stomatal responses to environmental stimuli. In this study, we devised a new method of transplanting, in which neither the mesophyll apoplast nor the epidermis apoplast directly contact the buffer solution. Utilising this method, we carefully re-examined whether the mesophyll signals play significant roles in stomatal responses to light and/or CO2 in Commelina communis. In this method, epidermal strips were prepared from the dark-treated leaves and immediately transplanted on the mesophyll segments pretreated in the light or dark at 100, 390 or 700 ppm CO2. We confirmed the presence of mesophyll signals inducing stomatal opening and closure.
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