Analysis of functional link between the trans-Golgi network and chloroplast

• Project/Area Number: 15K14538
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: Uemura Tomohiro 東京大学, 大学院理学系研究科(理学部), 助教 (90415092)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 葉緑体 / ＴＧＮ / TGN / 膜交通

Outline of Final Research Achievements

To understand how the TGN regulate the Chloroplast maintenance, we developed the system for observing Chloroplast dynamics after SA treatment. As the results, we obtained preliminary data of functional link between TGN and Chloroplast.

Research Products

• [Int'l Joint Research] Max Planck Institute(Germany)
• [Journal Article] A missense mutation in the NSF gene causes abnormal Golgi morphology in Arabidopsis thaliana. (2018)
  • Author(s): Sayuri Tanabashi, Keiko Shoda, Chieko Saito, Tomoaki Sakamoto, Tetsuya Kurata, Tomohiro Uemura, and Akihiko Nakano.
  • Journal Title: Cell Struct. Funct Volume: 43 Pages: 41-45
  • NAID: 130006565491
  • Peer Reviewed
• [Journal Article] Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana. (2017)
  • Author(s): Shanti S. Sharma, Kotaro Yamamoto, Kohei Hamaji, Miwa Ohnishi, Aya Anegawa, Shashi Sharma, Sveta Thakur, Vijay Kumar, Tomohiro Uemura, Akihiko Nakano, Tetsuro Mimura
  • Journal Title: Plant Physiol Biochem. Volume: 114 Pages: 29-37
  • DOI: 10.1016/j.plaphy.2017.02.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The trans-Golgi network and the Golgi stacks behave independently during regeneration after Brefeldin A treatment in tobacco BY-2 cells (2017)
  • Author(s): Ito, Y., Toyooka, K., Fujimoto, M., Ueda, T., Uemura, T. and Nakano A.
  • Journal Title: Plant Cell Phys. Volume: 印刷中 Issue: 4 Pages: 811-821
  • DOI: 10.1093/pcp/pcx028
  • Peer Reviewed / Open Access
• [Journal Article] Evolutionarily conserved BIL4 suppresses the degradation of brassinosteroid receptor BRI1 that regulates cell elongation. (2017)
  • Author(s): Ｙamagami, A., Saito, C., Nakazawa, M., Fujioka, S., Uemura, T., Matsui, M., Sakuta, M., Shinozaki, K., Osada, H., Nakano, A., Asami, T. and *Nakano, T.
  • Journal Title: Sci. Rep. Volume: 18 Pages: 5739-5739
  • Peer Reviewed / Open Access
• [Journal Article] Golgi entry core compartment functions as the COPII-independent scaffold for ER-Golgi transport in plant cells (2017)
  • Author(s): Ito Yoko、Uemura Tomohiro、Nakano Akihiko
  • Journal Title: Journal of Cell Science Volume: 131 Issue: 2
  • DOI: 10.1242/jcs.203893
  • Peer Reviewed
• [Journal Article] Distribution of RAB5-positive multivesicular endosomes and the <i>trans</i>-Golgi network in root meristematic cells of <i>Arabidopsis thaliana</i> (2016)
  • Author(s): Ito, E., Uemura, T., Ueda, T. (corresponding author), and Nakano, A.
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 4 Pages: 281-286
  • DOI: 10.5511/plantbiotechnology.16.0218a
  • NAID: 130005281074
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Physiological Roles of Plant Post-Golgi Transport Pathways in Membrane Trafficking (2016)
  • Author(s): Uemura, T.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 10 Pages: 2013-2019
  • DOI: 10.1093/pcp/pcw149
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] SNARE Molecules in Marchantia polymorpha: Unique and Conserved Features of the Membrane Fusion Machinery. (2016)
  • Author(s): Kanazawa, T., Era, A., Minamino, N., Shikano, Y., Fujimoto, M., Uemura, T., Nishihama, R., Yamato, KT., Ishizaki, K., Nishiyama, T., Kohchi, T., Nakano, A., Ueda, T.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 2 Pages: 307-24
  • DOI: 10.1093/pcp/pcv076
  • Peer Reviewed / Open Access
• [Journal Article] Distribution of RAB5-positive multivesicular endosomes and the trans-Golgi network in root meristematic cells of Arabidopsis thaliana (2016)
  • Author(s): Ito, E., Uemura, T. Ueda, T. and Nakano, A
  • Journal Title: Plant Biotechnology Volume: 印刷中
  • NAID: 130005281074
  • Peer Reviewed
• [Presentation] Functional zones and dynamics of trans-Golgi network in Arabidopsis (2017)
  • Author(s): Yutaro Shimizu, Yamato Komatsu, Yoko Ito, Junpei Takagi J, Kazuo Ebine, Takashi Ueda, Kazuo Kurokawa, Tomohiro Uemura, Akihiko Nakano.
  • Organizer: 20th European Network for Plant Endomembrane Research meeting
  • Int'l Joint Research
• [Presentation] 超解像ライブメージング観察による植物オルガネの動態と構造 (2017)
  • Author(s): 植村知博
  • Organizer: 異分野融合ワークショップ『細胞外から受ける情報のイメージングとそれを支える解析技術の最前線』
  • Place of Presentation: 奈良先端大 （奈良県生駒市）
  • Invited
• [Presentation] The dynamics and physiological roles of the TGN in plants (2016)
  • Author(s): Tomohiro Uemura
  • Organizer: rogress100: Second international symposium: Protein Trafficking and Intracellular Signaling of Plant and Fungal Cells
  • Place of Presentation: Hakata, Japan
  • Year and Date: 2016-02-08
  • Int'l Joint Research / Invited
• [Presentation] 植物におけるトランスゴルジネットワーク（ＴＧＮ）の動態と生理機能 (2015)
  • Author(s): 植村知博
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（神戸・兵庫）
  • Year and Date: 2015-12-02
  • Invited
• [Presentation] The dynamics of trans-Golgi network (TGN) in plants (2015)
  • Author(s): Tomohiro Uemura, Takashi Ueda, and Akihiko Nakano.
  • Organizer: 6th International Conference on Arabidopsis Research
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] 植物におけるTGNの動態と生理機能 (2015)
  • Author(s): 植村知博、中野明彦
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール堀船（江戸川区・東京）
  • Year and Date: 2015-06-30