Study of prototype histone codes for plant light/dark responses
| Project/Area Number |
15K14539
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Tanaka Kan 東京工業大学, 科学技術創成研究院, 教授 (60222113)
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| Co-Investigator(Kenkyū-buntansha) |
瀧 景子 東京工業大学, 科学技術創成研究院, 研究員 (50332284)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 光環境応答 / 原始紅藻 / ヒストンコード / 翻訳後修飾 / 転写調節 / Cyanidioschyzon merolae |
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| Outline of Final Research Achievements |
Using a primitive red alga Cyanidioschyzon merolae as the material, the presence and the dynamics of histone post-translational modifications in response to changes of light conditions were analyzed. We identified methylation and acetylation of histone H3K4 and H3K9, respectively, by specific monoclonal antibodies, and found these modifications accumulate at S phase and M phase of the algal cell cycle, respectively. In addition, we established a method to extract histone proteins from C. merolae cells, and also showed the possibility that homologs of plant photomorphogenic signaling factors, DET1 and COP1, are involved in the light-regulated transcriptional regulation in C. merolae.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016
Author(s)
Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 印刷中
Issue: 19
Pages: 5266-5304
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Expression of cyanobacterial acyl-ACP reductase elevates the triacylglycerol level in the red alga Cyanidioschyzon merolae.2015
Author(s)
Nobuko Sumiya, Yasuko Kawase, Junpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima
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Journal Title
Plant Cell Physiol.
Volume: 56
Issue: 10
Pages: 1962-1980
DOI
Related Report
Peer Reviewed
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