Study of prototype histone codes for plant light/dark responses

• Project/Area Number: 15K14539
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Tanaka Kan 東京工業大学, 科学技術創成研究院, 教授 (60222113)
• Co-Investigator(Kenkyū-buntansha): 瀧 景子 東京工業大学, 科学技術創成研究院, 研究員 (50332284)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 光環境応答 / 原始紅藻 / ヒストンコード / 翻訳後修飾 / 転写調節 / Cyanidioschyzon merolae

Outline of Final Research Achievements

Using a primitive red alga Cyanidioschyzon merolae as the material, the presence and the dynamics of histone post-translational modifications in response to changes of light conditions were analyzed. We identified methylation and acetylation of histone H3K4 and H3K9, respectively, by specific monoclonal antibodies, and found these modifications accumulate at S phase and M phase of the algal cell cycle, respectively. In addition, we established a method to extract histone proteins from C. merolae cells, and also showed the possibility that homologs of plant photomorphogenic signaling factors, DET1 and COP1, are involved in the light-regulated transcriptional regulation in C. merolae.

Research Products

• [Journal Article] Conserved two-component Hik34-Rre1 module directly activates heat-stress inducible transcription of major chaperone and other genes in Synechococcus elongatus PCC 7942 (2017)
  • Author(s): Ikki Kobayashi, Satoru Watanabe, Yu Kanesaki, Tomohiro Shimada, Hirofumi Yoshikawa and Kan Tanaka
  • Journal Title: Mol. Microbiol. Volume: 104 Issue: 2 Pages: 260-277
  • DOI: 10.1111/mmi.13624
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Construction of a rapamycin-susceptible strain of the unicellular red alga Cyanidioschyzon merolae for analysis of target of rapamycin (TOR) function (2017)
  • Author(s): Sousuke Imamura, Keiko Taki and Kan Tanaka
  • Journal Title: J. Gen. Appl. Microbiol. Volume: 印刷中
  • NAID: 130006206605
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of YbhA as the pyridoxal 5′-phosphate (PLP) phosphatase in Escherichia coli: Importance of PLP homeostasis on the bacterial growth (2017)
  • Author(s): Ryota Sugimoto, Natsumi Saito, Tomohiro Shimada and Kan Tanaka
  • Journal Title: J. Gen. Appl. Microbiol. Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii (2016)
  • Author(s): Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 印刷中 Issue: 19 Pages: 5266-5304
  • DOI: 10.1073/pnas.1525538113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcription factor DecR (YbaO) controls detoxification of L-cysteine in Escherichia coli. (2016)
  • Author(s): Tomohiro Shimada, Kan Tanaka, and Akira Ishihama
  • Journal Title: Microbiology Volume: 162 Issue: 9 Pages: 1698-1707
  • DOI: 10.1099/mic.0.000337
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Use of a Bacterial Luciferase Monitoring System to Estimate Real-time Dynamics of Intracellular Metabolism in Escherichia coli (2016)
  • Author(s): Tomohiro Shimada and Kan Tanaka
  • Journal Title: Appl. Environ. Microbiol. Volume: 82 Issue: 19 Pages: 5960-5968
  • DOI: 10.1128/aem.01400-16
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of tetrapyrrole biosynthetic genes explains abscisic acid-induced heme accumulation in the unicellular red alga Cyanidioschyzon merolae (2016)
  • Author(s): Yuki Kobayashi and Kan Tanaka
  • Journal Title: Front. Plant Sci. Volume: -
  • DOI: 10.3389/fpls.2016.01300
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Extraction and Measurement of Abscisic Acid in a Unicellular Red Alga Cyanidioschyzon merolae (2016)
  • Author(s): Yuki Kobayashi and Kan Tanaka
  • Journal Title: Bio-protocol Volume: 6 Issue: 23
  • DOI: 10.21769/bioprotoc.2033
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TOR (target of rapamycin) is a key regulator of triacylglycerol accumulation in microalgae. (2016)
  • Author(s): Sousuke Imamura, Yasuko Kawase, Ikki Kobayashi, Mie Shimojima, Hiroyuki Ohta, and Kan Tanaka
  • Journal Title: Plant Signal. Behav. Volume: in press Issue: 3 Pages: e1149285-e1149285
  • DOI: 10.1080/15592324.2016.1149285
  • Peer Reviewed
• [Journal Article] Abscisic acid participates in the control of cell-cycle initiation through heme homeostasis in the unicellular red alga Cyanidioschyzon merolae. (2016)
  • Author(s): Yuki Kobayashi, Hiroyuki Ando, Mitsumasa Hanaoka, and Kan Tanaka
  • Journal Title: Plant Cell Physiol. Volume: in press Issue: 5 Pages: 953-960
  • DOI: 10.1093/pcp/pcw054
  • Peer Reviewed / Open Access
• [Journal Article] Construction of a <i>URA5.3</i> deletion strain of the unicellular red alga <i>Cyanidioschyzon merolae</i>: A backgroundless host strain for transformation experiments (2015)
  • Author(s): Keiko Taki, Toshiyuki Sone, Yuki Kobayashi, Satoru Watanabe, Sousuke Imamura, and Kan Tanaka
  • Journal Title: The Journal of General and Applied Microbiology Volume: 61 Issue: 5 Pages: 211-214
  • DOI: 10.2323/jgam.61.211
  • NAID: 130005110096
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed / Open Access
• [Journal Article] A nitrogen source-dependent inducible and repressible gene expression system in the red alga Cyanidioschyzon merolae. (2015)
  • Author(s): Takayuki Fujiwara, Yu Kanesaki, Shunsuke Hirooka, Atsuko Era, Nobuko Sumiya, Hirofumi Yoshikawa, Kan Tanaka, Shin-ya Miyagishima
  • Journal Title: Front. Plant Sci. Volume: 6 Pages: 657-657
  • DOI: 10.3389/fpls.2015.00657
  • Peer Reviewed / Open Access
• [Journal Article] Expression of cyanobacterial acyl-ACP reductase elevates the triacylglycerol level in the red alga Cyanidioschyzon merolae. (2015)
  • Author(s): Nobuko Sumiya, Yasuko Kawase, Junpei Hayakawa, Mami Matsuda, Mami Nakamura, Atsuko Era, Kan Tanaka, Akihiko Kondo, Tomohisa Hasunuma, Sousuke Imamura, Shin-ya Miyagishima
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 10 Pages: 1962-1980
  • DOI: 10.1093/pcp/pcv120
  • Peer Reviewed
• [Journal Article] Target of rapamycin (TOR) plays a critical role in triacylglycerol accumulation in microalgae. (2015)
  • Author(s): Sousuke Imamura, Yasuko Kawase, Ikki Kobayashi, Toshiyuki Sone, Atsuko Era, Shin-ya Miyagishima, Mie Shimojima, Hiroyuki Ohta, and Kan Tanaka
  • Journal Title: Plant Mol. Biol. Volume: 89 Pages: 309-318
  • Peer Reviewed
• [Presentation] 大腸菌における低濃度翻訳阻害剤が引き起こす低温応答性遺伝子群の発現制御機構 (2017)
  • Author(s): ○川上賢佑、島田友裕、田中寛
  • Organizer: 第１１回 日本ゲノム微生物学会年会
  • Place of Presentation: 慶應義塾大学（湘南藤沢キャンパス）
  • Year and Date: 2017-03-03
• [Presentation] 単細胞紅藻シゾンにおける核遺伝子の光転写活性化機構の解析 (2017)
  • Author(s): ○吉川瞳子、小林勇気、瀧景子、今村壮輔、田中寛
  • Organizer: 第１１回 日本ゲノム微生物学会年会
  • Place of Presentation: 慶應義塾大学（湘南藤沢キャンパス）
  • Year and Date: 2017-03-03
• [Presentation] 大腸菌をモデルとしたエネルギー獲得代謝の切替え機構の解析 (2017)
  • Author(s): ○田中 寛
  • Organizer: 第１１回 日本ゲノム微生物学会年会・シンポジウム「微生物と代謝のフロンティア研究」
  • Place of Presentation: 慶應義塾大学（湘南藤沢キャンパス）
  • Invited
• [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおける多重遺伝子変異系の開発 (2017)
  • Author(s): ○竹村時空、小林勇気、今村壮輔、田中寛
  • Organizer: 第５８回 日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（郡元キャンパス）
• [Presentation] 紅藻Cyanidioschyzon merolaeにおけるABAシグナル伝達の解析 (2017)
  • Author(s): ○小林勇気、田中寛
  • Organizer: 第５８回 日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（郡元キャンパス）
• [Presentation] タイリングアレイによる原始紅藻シゾンの窒素応答転写因子CmMYB1の転写ターゲットの網羅的解析 (2017)
  • Author(s): ○瀧景子、曾根俊之、墨谷暢子、神崎陸、宮城島進也、今村壮輔、田中寛
  • Organizer: 第５８回 日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（郡元キャンパス）
• [Presentation] Biochemical characterization of tetrapyrrole binding pentatricopeptide-repeat (PPR) proteins in plastids (2017)
  • Author(s): ○Awasthi Saumya, Tomohiro Shimada, Kan Tanaka, Nobuyoshi Mochizuki, Tatsuru Masuda
  • Organizer: 第５８回 日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（郡元キャンパス）
• [Presentation] 単細胞紅藻C. merolaeのレトログレードシグナルに依存した転写制御 (2017)
  • Author(s): ○大原ひかる、安藤洸幸、小林勇気、今村壮輔、田中寛、五十嵐雅之、内海龍太郎、華岡光正
  • Organizer: 第５８回 日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（郡元キャンパス）
• [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942における強光応答の生物時計依存的な調節 (2017)
  • Author(s): ○鶴巻達大、田中寛
  • Organizer: 第５８回 日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（郡元キャンパス）
• [Presentation] 好塩性古細菌およびシアノバクテリアに保存された新規 ビオチン生合成酵素の機能解析 (2017)
  • Author(s): ○大石恵太、清水 哲、小林一幾、富田武郎、田中 寛、葛山智久、西山 真
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] 大腸菌におけるエネルギー代謝の切り替え機構 (2017)
  • Author(s): ○島田友裕、田中寛
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] 枯草菌 FoF1 ATP 合成酵素活性調節の生理的意義の解明 (2017)
  • Author(s): ○赤沼元気、澤田真帆、多賀名智明、島田友裕、田中寛、大坂夏木、吉川博文、河村富士夫、山田康之
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] 大腸菌におけるピリドキサール-5’-リン酸の恒常性における脱リン酸化酵素 YbhA の役割 (2017)
  • Author(s): 杉本良太、斎藤菜摘、島田友裕、田中寛
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] 単細胞紅藻 Cyanidioschyzon merolae の構成的プラ スミドトと抗生物質耐性マーカーの開発 (2016)
  • Author(s): ◯大沼みお, 藤原崇之, 井元祐太, 黒岩晴子, 宮城島 進也, 田中寛, 黒岩常祥
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2016-09-16
• [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942における生物時計と強光応答の関係性 (2016)
  • Author(s): ○鶴巻達大、田中 寛
  • Organizer: 第７回日本光合成学会年会
  • Place of Presentation: 東京理科大学（葛飾キャンパス）
  • Year and Date: 2016-05-27
• [Presentation] 単細胞紅藻類において暗誘導されるR1型MYB転写因子MYB2の機能解析 (2016)
  • Author(s): 河瀬泰子、今村壮輔、田中寛
  • Organizer: 第５７回 日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス（盛岡）
  • Year and Date: 2016-03-20
• [Presentation] 単細胞紅藻 Cyanidioschyzon merolaeにおけるABAの機能 (2016)
  • Author(s): 小林勇気、安藤洸幸、華岡光正、田中寛
  • Organizer: 第５７回 日本植物生理学会年会
  • Place of Presentation: 岩手大学上田キャンパス（盛岡）
  • Year and Date: 2016-03-18
• [Presentation] シアノバクテリアから葉緑体へ:共生進化を光環境応答から見る (2016)
  • Author(s): ○田中 寛
  • Organizer: 日本進化学会第18回東京大会・シンポジウムS６：植物の進化
  • Place of Presentation: 東京工業大学（大岡山）
  • Invited
• [Presentation] Conserved response regulators, RpaA and RpaB, coordinate circadian clock and high light response in cyanobacteria (2016)
  • Author(s): ◯Tatsuhiro Tsurumaki and Kan Tanaka
  • Organizer: Finnish-Japanese Symposium 2016
  • Place of Presentation: Lapland Hotel Riekonlinna, Saariselka, Finland
  • Int'l Joint Research
• [Presentation] Conserved Transcriptional Framework for Light Acclimation Processes: From Cyanobacteria to Chloroplasts (2016)
  • Author(s): Ikki Kobayashi, Tatsuhiro Tsurumaki, Rumi Suzuki and ◯Kan Tanaka
  • Organizer: Finnish-Japanese Symposium 2016
  • Place of Presentation: Lapland Hotel Riekonlinna, Saariselka, Finland
  • Int'l Joint Research / Invited
• [Presentation] 単細胞紅藻Cyanidioschyzon merolae（シゾン）の遺伝子改変ホスト株の分離 (2015)
  • Author(s): 瀧景子、曾根俊之、小林勇気、渡辺智、今村壮輔、田中寛
  • Organizer: 第38回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-03
• [Presentation] 紅藻 Cyanidioschyzon merolae におけるアブシシン酸の機能 (2015)
  • Author(s): 小林勇気、安藤洸幸、華岡光正、田中寛
  • Organizer: 第38回 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-02
• [Presentation] Abscisic acid signaling in a unicellular red alga (2015)
  • Author(s): Yuki Kobayashi and Kan Tanaka
  • Organizer: Yamada Symposium, Dynamics and Regulation of Photosynthesis
  • Place of Presentation: 奈良市
  • Year and Date: 2015-10-30
  • Int'l Joint Research / Invited