Analysis of polyspermy block system based on multiple pollen tube attraction.

• Project/Area Number: 15K14541
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Yokohama City University (2016-2017); Nagoya University (2015)
• Principal Investigator: Maruyama Daisuke 横浜市立大学, 木原生物学研究所, 助教 (80724111)
• Research Collaborator: HIGASHIYAMA Tetsuya ; KAWASHIMA Tomokazu ; Berger Frederic
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 重複受精 / 花粉管 / 卵細胞 / 中央細胞 / 多花粉管拒否 / 多精拒否 / シロイヌナズナ / 助細胞胚乳融合 / 精細胞 / 胚乳 / 助細胞 / 細胞融合

Outline of Final Research Achievements

Zygote of flowering plants prevents multiple fertilizations caused by additional pair of sperm cells from second pollen tube. To examine the mechanism of polyspermy block, we analyzed patterns of released pollen tube contents (PTC) including sperm cells in an Arabidopsis mutant that frequently attracts second pollen tube. Contrary to our expectation, fluorescent signals from the PTC were frequently found in the endosperm but not in the fertilization zone. As this unique phenomenon seems to avoid polyspermy through a rerouting of sperm destination, we further analyzed other mutants that attract multiple pollen tubes and found possible involvement of synergid-endosperm fusion (SE fusion) during early embryogenesis. To prove the relevance of SE fusion in the unusual PTC reception, we tried to isolate mutant plants defective in the SE fusion and finally obtained one.

Research Products

• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research] Kentucky University(米国)
• [Journal Article] A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm. (2018)
  • Author(s): Motomura K, Kawashima T, Berger F, Kinoshita T, Higashiyama T, Maruyama D.
  • Journal Title: J Cell Sci. Volume: 131 Issue: 2 Pages: 1-6
  • DOI: 10.1242/jcs.204123
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] RETINOBLASTOMA RELATED1 mediates germline entry in Arabidopsis. (2017)
  • Author(s): Zhao, X., Bramsiepe, J., Van Durme, M., Komaki, S., Prusicki, M.A., Maruyama, D., Forner, J., Medzihradszky, A., Wijnker, E., Harashima, H., Lu, Y., Schmidt, A., Guthorl, D., Logrono, R.S., Guan, Y., Pochon, G., Grossniklaus, U., Laux, T., Higashiyama, T., Lohmann, J.U., Nowack, M.K., Schnittger, A.
  • Journal Title: Science Volume: 356 Issue: 6336
  • DOI: 10.1126/science.aaf6532
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines (2017)
  • Author(s): Mina Ohtsu, Yoshikatsu Sato, Daisuke Kurihara, Takuya Suzaki, Masayoshi Kawaguchi, Daisuke Maruyama, Tetsuya Higashiyama
  • Journal Title: Protoplasma Volume: 印刷中 Issue: 6 Pages: 2107-2115
  • DOI: 10.1007/s00709-017-1105-0
  • NAID: 120006382262
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization. (2016)
  • Author(s): Kasahara R., Notaguchi M., Nagahara S., Suzuki T., Susaki D., Honma Y., Maruyama D., Higashiyama T.
  • Journal Title: Science Advances Volume: 2 Issue: 10 Pages: 1-7
  • DOI: 10.1126/sciadv.1600554
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The end of temptation: the elimination of persistent synergid cell identity (2016)
  • Author(s): Daisuke Maruyama, Tetsuya Higashiyama
  • Journal Title: Current Opinion in Plant Biology Volume: 34 Pages: 122-126
  • DOI: 10.1016/j.pbi.2016.10.011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cell fusion and nuclear fusion in plants (2016)
  • Author(s): Daisuke Maruyama, Tetsuya Higashiyama
  • Journal Title: Seminars in Cell & Developmental Biology Volume: 60 Pages: 127-135
  • DOI: 10.1016/j.semcdb.2016.07.024
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Fertilization-independent Cell-fusion between the Synergid and Central Cell in the Polycomb Mutant (2016)
  • Author(s): Kazuki Motomura, Frederic Berger, Tomokazu Kawashima, Tetsu Kinoshita, Tetsuya Higashiyama, Daisuke Maruyama
  • Journal Title: Cell Structure and Function Volume: 41 Issue: 2 Pages: 121-125
  • DOI: 10.1247/csf.16010
  • NAID: 130005405450
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism. (2015)
  • Author(s): 丸山大輔、Ronny Völz、竹内英憲、森稔幸、井川智子、栗原大輔、河島友和、植田美那子、伊藤正樹、梅田正明、西川周一、Rita Groß-Hardt、東山哲也
  • Journal Title: Cell Volume: 未定 Issue: 4 Pages: 907-918
  • DOI: 10.1016/j.cell.2015.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Selective Elimination in Multinucleate Cells. (2015)
  • Author(s): Daisuke Maruyama, Tomokazu Kawashima, Tetsuya Higashiyama
  • Journal Title: Oncotarget Volume: 6 Issue: 31 Pages: 30447-30448
  • DOI: 10.18632/oncotarget.5450
  • Peer Reviewed
• [Journal Article] BiP3 supports the early stages of female gametogenesis in the absence of BiP1 and BiP2 in Arabidopsis thaliana. (2015)
  • Author(s): Daisuke Maruyama, Toshiya Endo, Shuh-ichi Nishikawa
  • Journal Title: Plant Signaling & Behavior Volume: 10 Issue: 7 Pages: e1035853-e1035853
  • DOI: 10.1080/15592324.2015.1035853
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Analysis of the structure surrounding the egg cell in Arabidopsis thaliana. (2018)
  • Author(s): Susaki, D., Oi, T., Tomomi S., Izumi, R., Enomoto, S., Arai, S., Kinoshita, T., Maruyama., D.
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] Reception of pollen tube contents by the endosperm. (2018)
  • Author(s): Daisuke Maruyama, Daichi Susaki, Tetsuya Higashiyama Tetsu Kinoshita
  • Organizer: The 25th International Congress on Sexual Plant Reproduction
• [Presentation] 助細胞は精細胞の放出先の位置を制御する微小流路として機能する (2017)
  • Author(s): 須崎 大地、東山 哲也、木下 哲、丸山 大輔
  • Organizer: 日本植物学会第81回大会
• [Presentation] 植物システムを支える運命の分岐点 (2016)
  • Author(s): 丸山大輔、東山哲也
  • Organizer: 日本細胞生物学会
  • Place of Presentation: テルサ京都（京都府京都市）
  • Year and Date: 2016-06-15
• [Presentation] 花粉管誘引停止を引き起こす助細胞胚乳融合の解析 (2015)
  • Author(s): 丸山大輔, 武内秀憲, 森稔幸, 井川智子, 河島友和, Frédéric Berger & 東山哲也
  • Organizer: BMB2015 シンポジウム「受精を支える分子とそれを取り巻く分子環境」
  • Place of Presentation: 神戸市（神戸ポートアイランド）
  • Year and Date: 2015-12-01
• [Presentation] 新奇の植物細胞融合による花粉管誘引停止機構の発見 (2015)
  • Author(s): 丸山大輔, Voelz. R., 武内秀憲, 森稔幸, 井川智子, 河島友和, 西川周一, Gross-Hardt R. & 東山哲也
  • Organizer: 日本植物学会第79回大会シンポジウム「花から種子へ ― 稔りを巡る研究の新展開」
  • Place of Presentation: 新潟市（朱鷺メッセ）
  • Year and Date: 2015-09-06
• [Presentation] 細胞融合による急速な助細胞排除機構の解析 (2015)
  • Author(s): Daisuke Maruyama, Ronny Voelz, Hidenori Takeuchi, Toshiyuki Mori, Tomoko Igawa, Daisuke Kurihara, Tomokazu Kawashima, Minako Ueda, Masaki Ito, Masaaki Umeda, Shuh-ichi Nishikawa, Rita Gross-Hardt, Tetsuya Higashiyama
  • Organizer: 日本植物形態学会第２７回大会
  • Place of Presentation: 新潟市（朱鷺メッセ）
  • Year and Date: 2015-09-05
• [Presentation] 花粉管誘引を停止させる助細胞の排除は細胞融合によって起こる (2015)
  • Author(s): 丸山大輔, Voelz R., 武内秀憲, 森稔幸, 井川智子, 河島友和, 西川周一, Gross-Hardt R. & 東山哲也
  • Organizer: 第27回高遠シンポジウム
  • Place of Presentation: 滋賀県大津市（延暦寺会館）
  • Year and Date: 2015-08-26
• [Presentation] 細胞融合による急速な助細胞排除機構の解析 (2015)
  • Author(s): 丸山大輔, Voelz. R., 武内秀憲, 森稔幸, 井川智子, 河島友和, 西川周一, Gross-Hardt R. & 東山哲也
  • Organizer: 第６７回日本細胞生物学会大会
  • Place of Presentation: 東京都（タワー船堀）
  • Year and Date: 2015-06-30
• [Book] Plant Germline Development (2017)
  • Author(s): Susaki, D., Maruyama, D., Yelagandula, R., Berger, F., Kawashima, T.
  • Publisher: Humana Press, New York, NY
  • ISBN: 9781493972869
• [Remarks] 植物の受精における細胞融合による残存助細胞の迅速な排除
  • URL: http://first.lifesciencedb.jp/archives/10061.