Chemical library screening to identify novel key regulators of organelle distribution in plants
| Project/Area Number |
15K14542
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
SATO YOSHIKATSU 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (30414014)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Ayato 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (10512428)
KUWATA Keiko 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (70624352)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 化合物ライブラリースクリーニング / 葉緑体 / オルガネラ分布 / オルガネラ |
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| Outline of Final Research Achievements |
Proper distribution of organelles is essential for proper cell function in eukaryotic cells. In this study, we performed chemical library screening to identify novel key regulators of organelle distribution in plants. As a result of high-throughput but careful screening design, we obtained 9 candidates from 20,000 chemicals, which can be divided into 4 groups by structure-activity relationships. We focused 1 of these groups and evaluated by time-lapse analysis. Then, we confirmed 5 candidates having common structures. Furthermore, we succeeded to identify the essential and non-essential part from hit compounds and to synthesize the dimer compound having strong effect on organelle distribution. Therefore, we conclude that we could obtain important tools and information to elucidate the basic mechanism of organelle distribution in plants.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote.2016
Author(s)
Kimata, Y., Higaki, T., Kawashima, T., Kurihara, D., Sato, Y., Yamada, T., Hasezawa, S., Berger, F., Higashiyama, T., and Ueda, M.
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 113
Issue: 49
Pages: 14157-14162
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Live-cell imaging of zygote polarization in Arabidopsis2017
Author(s)
Yusuke Kimata, Takumi Higaki, Tomokazu Kawashima, Daisuke Kurihara, Yoshikatsu Sato, Tomomi Yamada, Seiichiro Hasezawa, Frederic Berger, Tetsuya Higashiyama, Minako Ueda
Organizer
The 1st ABiS Symposium: Towards the Future of Advanced Bioimaging for Life Sciences
Place of Presentation
岡崎コンファレンスセンター
Year and Date
2017-02-19
Related Report
Int'l Joint Research
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[Presentation] 植物受精卵のライブイメージング:細胞極性とは何か？2016
Author(s)
木全祐資, 桧垣匠, 河島友和, 栗原大輔, 佐藤良勝, 山田朋美, 馳澤盛一郎, Frederic Berger, 東山哲也, 植田美那子
Organizer
日本植物学会第80回大会
Place of Presentation
沖縄コンベンションセンター
Year and Date
2016-09-16
Related Report
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