| Project/Area Number |
15K14542
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Plant molecular biology/Plant physiology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
SATO YOSHIKATSU 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (30414014)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SATO Ayato 名古屋大学, トランスフォーマティブ生命分子研究所, 特任准教授 (10512428)
KUWATA Keiko 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (70624352)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
|---|
| Keywords | 化合物ライブラリースクリーニング / 葉緑体 / オルガネラ分布 / オルガネラ |
|---|
| Outline of Final Research Achievements |
Proper distribution of organelles is essential for proper cell function in eukaryotic cells. In this study, we performed chemical library screening to identify novel key regulators of organelle distribution in plants. As a result of high-throughput but careful screening design, we obtained 9 candidates from 20,000 chemicals, which can be divided into 4 groups by structure-activity relationships. We focused 1 of these groups and evaluated by time-lapse analysis. Then, we confirmed 5 candidates having common structures. Furthermore, we succeeded to identify the essential and non-essential part from hit compounds and to synthesize the dimer compound having strong effect on organelle distribution. Therefore, we conclude that we could obtain important tools and information to elucidate the basic mechanism of organelle distribution in plants.
|
