Molecular analysis of plant kinetochore

• Project/Area Number: 15K14545
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Tamura Kentaro 京都大学, 理学研究科, 助教 (40378609)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: シロイヌナズナ / キネトコア / タンパク質複合体 / インタラクトーム / GFP / クロマチン / イメージング / ヒストン / プロテオミクス / 相互作用

Outline of Final Research Achievements

Kinetochores are the fundamental link between chromosomal domains called centromeres and spindle microtubules in all eukaryotes. This link, composed by multiprotein complexes, is essential for precise chromosome segregation into daughter cells and thus keeps genetic stability. In yeast and animal cells, many components of kinetochores have been identified and characterised. On the contrary, in plants, number of identified kinetochore protein is largely limited. To identify comprehensively the kinetochore components in plants, we performed interactome analysis in Arabidopsis plants that stably express centromere proteins fused with a fluorescent protein. We found that newly identified proteins, which include kinase- associated protein and unknown proteins, are likely associated with plant kinetochore structure. Together, these findings contribute to a unified view of how plant kinetochore functions as a regulator of chromatin segregation.

Research Products

• [Int'l Joint Research] Institut Jean-Pierre Bourgin (IJPB)/Clermont University(France)
• [Journal Article] Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana (2017)
  • Author(s): Kentaro Tamura, Y. Fukao, N. Hatsugai, F. Katagiri, I. Hara-Nishimura
  • Journal Title: Nucleus Volume: 印刷中 Issue: 3 Pages: 301-311
  • DOI: 10.1080/19491034.2017.1279774
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Exploring the protein composition of the plant nuclear envelope (2017)
  • Author(s): X. Zhou, Kentaro Tamura, K. Graumann, and I. Meier
  • Journal Title: Methods Mol Biol. Volume: 1411 Pages: 45-66
  • DOI: 10.1007/978-1-4939-3530-7_2
  • ISBN: 9781493935284, 9781493935307
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Decreased expression of a gene caused by a T-DNA insertion in an adjacent gene in Arabidopsis (2016)
  • Author(s): K. Tamura, T. Kawabayashi, T. Shikanai, and I. Hara-Nishimura
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0147911-e0147911
  • DOI: 10.1371/journal.pone.0147911
  • NAID: 120005743647
  • Peer Reviewed / Open Access
• [Journal Article] Plant nuclei move to escape ultraviolet-induced DNA damage and cell death. (2016)
  • Author(s): Iwabuchi K., Hidema J., Tamura K., Takagi S., Hara-Nishimura I.
  • Journal Title: Plant Physiology Volume: 170 Issue: 2 Pages: 678-685
  • DOI: 10.1104/pp.15.01400
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis. (2016)
  • Author(s): Ueda, H., Yokota, E., Kuwata, K., Kutsuna, N., Mano, S., Shimada, T., Tamura, K., Stefano, G., Fukao, Y., Brandizzi, F., Shimmen, T., Nishimura, M., Hara-Nishimura, I.
  • Journal Title: Plant Physiol. Volume: 170 Issue: 2 Pages: 867-880
  • DOI: 10.1104/pp.15.01172
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functions of plant-specific myosin XI: from intracellular motility to plant postures. (2015)
  • Author(s): Ueda, H., Tamura, K., Hara-Nishimura, I.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 28 Pages: 30-38
  • DOI: 10.1016/j.pbi.2015.08.006
  • NAID: 120005676685
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recent advances in understanding plant nuclear envelope proteins involved in nuclear morphology (2015)
  • Author(s): Kentaro Tamura, Chieko Goto and Ikuko Hara-Nishimura
  • Journal Title: Journal of Experimental Botany Volume: 66 Issue: 6 Pages: 1641-1647
  • DOI: 10.1093/jxb/erv036
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of organ straightening and plant posture by an actin-myosin XI cytoskeleton (2015)
  • Author(s): Okamoto, K., Ueda, H., Shimada, T., Tamura, K., Kato, T., Tasaka, M., Morita, M.T., and Hara-Nishimura, I.
  • Journal Title: Nature Plants Volume: 1 Issue: 4 Pages: 1-6
  • DOI: 10.1038/nplants.2015.31
  • NAID: 120005593639
  • Description: 【掲載確定】
  • Peer Reviewed
• [Presentation] Nuclear Movement and Shape Are Controlled by Nuclear Membrane Myosin XI-i (2016)
  • Author(s): Kentaro Tamura and Ikuko Hara-Nishimura
  • Organizer: 日本植物生理学会
  • Place of Presentation: 盛岡市
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] Emerging roles for nuclear membrane in signalling pathway in higher plants (2016)
  • Author(s): Kentaro Tamura
  • Organizer: Society for Experimental Biology Annual Main Meeting
  • Place of Presentation: Brighton, United Kingdom
  • Int'l Joint Research / Invited
• [Presentation] The unique nucleocytoplasmic linkage in plants (2015)
  • Author(s): Kentaro Tamura
  • Organizer: BMB2015
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-04
• [Presentation] Molecular relationships between the plant-specific nuclear envelope proteins (2015)
  • Author(s): Kentaro Tamura
  • Organizer: IPNC2015
  • Place of Presentation: Olomouc, Czech Republic
  • Year and Date: 2015-07-04
  • Int'l Joint Research / Invited