Molecular analysis of plant kinetochore
| Project/Area Number |
15K14545
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シロイヌナズナ / キネトコア / タンパク質複合体 / インタラクトーム / GFP / クロマチン / イメージング / ヒストン / プロテオミクス / 相互作用 |
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| Outline of Final Research Achievements |
Kinetochores are the fundamental link between chromosomal domains called centromeres and spindle microtubules in all eukaryotes. This link, composed by multiprotein complexes, is essential for precise chromosome segregation into daughter cells and thus keeps genetic stability. In yeast and animal cells, many components of kinetochores have been identified and characterised. On the contrary, in plants, number of identified kinetochore protein is largely limited. To identify comprehensively the kinetochore components in plants, we performed interactome analysis in Arabidopsis plants that stably express centromere proteins fused with a fluorescent protein. We found that newly identified proteins, which include kinase- associated protein and unknown proteins, are likely associated with plant kinetochore structure. Together, these findings contribute to a unified view of how plant kinetochore functions as a regulator of chromatin segregation.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis.2016
Author(s)
Ueda, H., Yokota, E., Kuwata, K., Kutsuna, N., Mano, S., Shimada, T., Tamura, K., Stefano, G., Fukao, Y., Brandizzi, F., Shimmen, T., Nishimura, M., Hara-Nishimura, I.
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Journal Title
Plant Physiol.
Volume: 170
Issue: 2
Pages: 867-880
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Regulation of organ straightening and plant posture by an actin-myosin XI cytoskeleton2015
Author(s)
Okamoto, K., Ueda, H., Shimada, T., Tamura, K., Kato, T., Tasaka, M., Morita, M.T., and Hara-Nishimura, I.
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Journal Title
Nature Plants
Volume: 1
Issue: 4
Pages: 1-6
DOI
NAID
Description
【掲載確定】
Related Report
Peer Reviewed
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