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Development of a microscope system that enables time-lapse imaging of cellular behavior in growing roots

Research Project

Project/Area Number 15K14548
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Nakajima Keiji  奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80273853)

Co-Investigator(Renkei-kenkyūsha) MIYASHIMA Shunsuke  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (20727169)
GOH Tatsuaki  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80511419)
FURUTA Kaori  奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (10746655)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords植物 / 根 / 顕微鏡 / タイムラプス / シロイヌナズナ
Outline of Final Research Achievements

Continuous observation of cell behavior and gene expression dynamics at the tip of growing roots is essential to investigate the regulation of root and plant growth. It had been, however, very challenging, as the tip of extending roots easily go out of a microscopic field. Using a microscope with a vertically oriented, motor-driven sample stage, we developed a system that can track the tip of growing roots for several days. Using this system, we could successfully follow an entire process of cell detachment in the Arabidopsis root cap.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2017 2016

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] シロイヌナズナ根冠細胞の分化と剥離の動態解析2017

    • Author(s)
      郷達明、上野皓輝、小園紗希、神谷雅子、金鍾明、遠藤高帆、宮島俊介、中島敬二
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県・鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Mechanisms regulating root cap differentiation and functions2016

    • Author(s)
      Keiji Nakajima
    • Organizer
      Japan-Taiwan bilateral minisymposium
    • Place of Presentation
      Taipei (Taiwan)
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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