Development of a microscope system that enables time-lapse imaging of cellular behavior in growing roots
| Project/Area Number |
15K14548
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Nakajima Keiji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80273853)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYASHIMA Shunsuke 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (20727169)
GOH Tatsuaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80511419)
FURUTA Kaori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (10746655)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 植物 / 根 / 顕微鏡 / タイムラプス / シロイヌナズナ |
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| Outline of Final Research Achievements |
Continuous observation of cell behavior and gene expression dynamics at the tip of growing roots is essential to investigate the regulation of root and plant growth. It had been, however, very challenging, as the tip of extending roots easily go out of a microscopic field. Using a microscope with a vertically oriented, motor-driven sample stage, we developed a system that can track the tip of growing roots for several days. Using this system, we could successfully follow an entire process of cell detachment in the Arabidopsis root cap.
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Report
(3 results)
Research Products
(2 results)
