| Project/Area Number |
15K14550
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMATO Masahide 千葉大学, 教育学部, 准教授 (00571788)
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| Research Collaborator |
IMAIZUMI Haruko (ANRAKU Haruko)
MIURA Chihiro
YAMAMOTO Tatsuki
FURUI Yuki
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ラン / 菌根共生 / トランスクリプトーム / 菌寄生性 / 共生菌 / 植物 / 菌類 / ゲノム / 進化 / 遺伝子 |
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| Outline of Final Research Achievements |
The mycorrhizal symbiosis in orchids has a feature that carbon is provided by mycorrhizal fungi, whose nutrition mode is termed as mycoheterotrophy and opposite to the general mutual symbioses. It has been reported that the genes of common symbiotic pathway are required for mutualistic plant-mycorrhizal symbioses, but the information about the molecular mechanisms of the relationship between orchids and their mycorrhizal fungi is limited. In this study, we conducted the transcriptome analysis and expression analysis of marker genes for mutual symbiosis using symbiotic germination of Bletilla striata (Orchidaceae) and the complementation experiment using CCaMK, which is a key gene for common symbiotic pathway. Our results partially demonstrated that the establishment of mycoheterotrophic symbiosis between orchids and mycorrhizal fungi require the genes of common symbiotic pathway necessary for mutualistic plant-mycorrhizal symbioses.
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