Does the establishment of mycoheterotrophic symbiosis between orchids and mycorrhizal fungi require the genes of common symbiotic pathway necessary for mutualistic plant-mycorrhizal symbioses?
Project/Area Number |
15K14550
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Tottori University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
YAMATO Masahide 千葉大学, 教育学部, 准教授 (00571788)
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Research Collaborator |
IMAIZUMI Haruko (ANRAKU Haruko)
MIURA Chihiro
YAMAMOTO Tatsuki
FURUI Yuki
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | ラン / 菌根共生 / トランスクリプトーム / 菌寄生性 / 共生菌 / 植物 / 菌類 / ゲノム / 進化 / 遺伝子 |
Outline of Final Research Achievements |
The mycorrhizal symbiosis in orchids has a feature that carbon is provided by mycorrhizal fungi, whose nutrition mode is termed as mycoheterotrophy and opposite to the general mutual symbioses. It has been reported that the genes of common symbiotic pathway are required for mutualistic plant-mycorrhizal symbioses, but the information about the molecular mechanisms of the relationship between orchids and their mycorrhizal fungi is limited. In this study, we conducted the transcriptome analysis and expression analysis of marker genes for mutual symbiosis using symbiotic germination of Bletilla striata (Orchidaceae) and the complementation experiment using CCaMK, which is a key gene for common symbiotic pathway. Our results partially demonstrated that the establishment of mycoheterotrophic symbiosis between orchids and mycorrhizal fungi require the genes of common symbiotic pathway necessary for mutualistic plant-mycorrhizal symbioses.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] The mycoheterotrophic symbiosis between orchids and mycorrhizal fungi possesses major components shared with mutualistic plant-mycorrhizal symbioses.2018
Author(s)
Miura, C., Yamaguchi, K., Miyahara, R., Yamamoto, T., Fuji, M., Yagame, T., Imaizumi-Anraku, H., Yamato, M., Shigenobu, S. and Kaminaka, H.
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Journal Title
Molecular Plant Microbe Interactions
Volume: 31
Issue: 10
Pages: 1032-1047
DOI
Related Report
Peer Reviewed
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[Journal Article] Quantitative evaluation of protocorm growth and fungal colonization in Bletilla striata (Orchidaceae) reveals less-productive symbiosis with a non-native symbiotic fungus2017
Author(s)
Yamamoto, T., Miura, C., Fuji, M., Nagata, S., Otani, Y., Yagame, T., Yamato, M. and Kaminaka, H
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Journal Title
BMC Plant Biol.
Volume: 17
Issue: 1
Pages: 50-50
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Comparison of green and albino individuals of the partially mycoheterotrophic orchid Epipactis helleborine on molecular identities of mycorrhizal fungi, nutritional modes, and gene expression in mycorrhizal roots2017
Author(s)
Suetsugu, K., Yamato, M., Miura, C., Yamaguchi, K., Takahashi, K., Ida, Y., Shigenobu, S. and Kaminaka, H.
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Journal Title
Mol. Ecol.
Volume: 26
Issue: 6
Pages: 1652-1669
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner2016
Author(s)
Takagi, H., Ishiga Y., Watanabe W., Konishi T., Egusa M., Akiyoshi N., Matsuura T., Mori I. C., Hirayama T., Kaminaka H., Shimada H. and Sakamoto A
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Journal Title
J. Ext. Bot.
Volume: 67
Issue: 8
Pages: 2519-2532
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Chitin nanofiber elucidates the elicitor activity of polymeric chitin in plants2015
Author(s)
Egusa, M., Matsui, H., Urakami, T., Okuda, S., Ifuku, S., Nakagami, H. and Kaminaka, H.
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Journal Title
frontiers in Plant Science
Volume: 6
Pages: 1098-1098
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Characterization of chitosan nanofiber sheets for antifungal application2015
Author(s)
Egusa, M., Iwamoto, R., Izawa, H., Morimoto, M., Saimoto, H., Kaminaka, H. and Ifuku, S.
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Journal Title
Int. J. Mol. Sci.
Volume: 16
Issue: 11
Pages: 26202-26210
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Involvement of auxin and gibberellic acid in the regulation of mycorrhizal symbiosis in Bletilla striata (Orchidaceae)2016
Author(s)
Chihiro Miura, Tatsuki Yamamoto, Masahide Yamato, Katsushi Yamaguchi, Shotaro Nagata, Yuria Otani, Hisayo Asao, Miwako Matsumoto, Takahiro Yagame, Shuji Shigenobu, Hironori Kaminaka
Organizer
XVII International Congress of Molecular Plant-Microbe Interactions
Place of Presentation
ポートランド(米国)
Year and Date
2016-07-17
Related Report
Int'l Joint Research
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[Presentation] Involvement of auxin and gibberellic acid in the regulation of mycorrhizal symbiosis in Bletilla striata (Orchidaceae)2016
Author(s)
Chihiro Miura, Tatsuki Yamamoto, Masahide Yamato, Katsushi Yamaguchi, Shotaro Nagata, Yuria Otani, Hisayo Asao, Miwako Matsumoto, Takahiro Yagame, Shuji Shigenobu, Hironori Kaminaka
Organizer
XVII International Congress of Molecular Plant-Microbe Interactions
Place of Presentation
Portland, Oregon, USA
Year and Date
2016-07-17
Related Report
Int'l Joint Research
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[Presentation] Involvement of auxin and gibberellic acid in the regulation of mycorrhizal symbiosis in Bletilla striata (Orchidaceae)2016
Author(s)
Chihiro Miura, Tatsuki Yamamoto, Masahide Yamato, Katsushi Yamaguchi, Shotaro Nagata, Yuria Otani, Hisayo Asao, Miwako Matsumoto, Takahiro Yagame, Shuji Shigenobu, Hironori Kaminaka
Organizer
第57回日本植物生理学会年会
Place of Presentation
岩手大学
Year and Date
2016-03-18
Related Report
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