Application of energy conversion apparatus consisting of photosystem I complex fused with tailor-made peptide tag
Project/Area Number |
15K14551
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Okayama University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
IMANAKA Hiroyuki 岡山大学, 大学院自然科学研究科, 助教 (10379711)
近藤 正晴 名古屋工業大学, 工学研究科, 助教 (20571219)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 光合成 / 光化学系I / 葉緑体形質転換 / 葉緑体電池 / アフィニティータグ / ペプチドタグ / 光化学系I複合体 / タンパク質工学 / 光電池 / クラミドモナス / 光化学系1複合体 |
Outline of Final Research Achievements |
We have developed a chloroplast battery cell which consists of photosynthetic proteins bound to an electrode and produces electricity under illumination. Peptide tags (Au-tags), which have an affinity to gold electrodes, have been screened by the bio-panning method using T7 bacteriophage. We genetically fused the resulting tags to one of the photosystem I (PSI) reaction center proteins and expressed it in the chloroplast of the green alga Chlamydomonas reinhardtii. The PSI complex containing an Ag-tag was purified and was bound to the electrode, resulting in the chloroplast battery cell. We measured electricity of the chloroplast battery cells with wild-type and Au-tag fused PSI complexes in the presence of artificial electron donors under illumination. It was found that only the chloroplast battery cell of Au-tagged PSI complex exhibited electricity. In conclusion, our strategy to use affinity peptide tag to bind PSI complex on the electrode is effective and useful.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii2016
Author(s)
L. Wang, T. Yamano, S. Takane, Y. Niikawa, C. Toyokawa, S. Ozawa, R. Tokutsu, Y. Takahashi, J. Minagawa, Y. Kanesaki, H. Yoshikawa, and H. Fukuzawa
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 113
Issue: 44
Pages: 12586-12591
DOI
NAID
Related Report
Peer Reviewed / Open Access
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