| Project/Area Number |
15K14551
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IMANAKA Hiroyuki 岡山大学, 大学院自然科学研究科, 助教 (10379711)
近藤 正晴 名古屋工業大学, 工学研究科, 助教 (20571219)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 光合成 / 光化学系I / 葉緑体形質転換 / 葉緑体電池 / アフィニティータグ / ペプチドタグ / 光化学系I複合体 / タンパク質工学 / 光電池 / クラミドモナス / 光化学系1複合体 |
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| Outline of Final Research Achievements |
We have developed a chloroplast battery cell which consists of photosynthetic proteins bound to an electrode and produces electricity under illumination. Peptide tags (Au-tags), which have an affinity to gold electrodes, have been screened by the bio-panning method using T7 bacteriophage. We genetically fused the resulting tags to one of the photosystem I (PSI) reaction center proteins and expressed it in the chloroplast of the green alga Chlamydomonas reinhardtii. The PSI complex containing an Ag-tag was purified and was bound to the electrode, resulting in the chloroplast battery cell. We measured electricity of the chloroplast battery cells with wild-type and Au-tag fused PSI complexes in the presence of artificial electron donors under illumination. It was found that only the chloroplast battery cell of Au-tagged PSI complex exhibited electricity. In conclusion, our strategy to use affinity peptide tag to bind PSI complex on the electrode is effective and useful.
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