Approach of phylogenic evolution to brain with focusing on sexually dimorphic nucleus

• Project/Area Number: 15K14556
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Saitama University
• Principal Investigator: TSUKAHARA Shinji 埼玉大学, 理工学研究科, 准教授 (90318824)
• Co-Investigator(Kenkyū-buntansha): 前川 文彦 国立研究開発法人国立環境研究所, 環境リスク・健康研究センター, 主任研究員 (40382866)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 性的二型核 / 種差 / カルビンディン / 性差

Outline of Final Research Achievements

Nuclei exhibiting morphological sex differences are termed sexually dimorphic nuclei (SDNs). The SDNs have been identified in the brain of several animal species, although the difference and similarity in the SDNs among animal species are poorly understood. In the current study, we examined the brain of mice, musk shrews, common marmosets, and quails to compare the SDNs among the animal species. There were species differences in the SDNs among mice, musk shrews, and common marmosets. However, many similarities of the SDNs were found among them. When compared quails to mice, musk shrews, and common marmosets, striking differences were found between the avian model and the mammalian models.

Research Products

• [Journal Article] Moxd1 Is a Marker for Sexual Dimorphism in the Medial Preoptic Area, Bed Nucleus of the Stria Terminalis and Medial Amygdala (2017)
  • Author(s): Tsuneoka Y and Funato H et al.
  • Journal Title: Front Neuroanat Volume: 11 Pages: 26-26
  • DOI: 10.3389/fnana.2017.00026
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Exposure of BALB/c Mice to Diesel Engine Exhaust Origin Secondary Organic Aerosol (DE-SOA) during the Developmental Stages Impairs the Social Behavior in Adult Life of the Males (2016)
  • Author(s): Tin-Tin Win-Shwe, Chaw Kyi-Tha-Thu, Yadanar Moe, Yuji Fujitani, Shinji Tsukahara, Seishiro Hirano
  • Journal Title: Front. Neurosci. Volume: 9 Pages: 524-524
  • DOI: 10.3389/fnins.2015.00524
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Prenatal Exposure to Arsenic Impairs Behavioral Flexibility and Cortical Structure in Mice (2016)
  • Author(s): Aung KH, Kyi-Tha-Thu C, Sano K, Nakamura K, Tanoue A, Nohara K, Kakeyama M, Tohyama C, Tsukahara S, Maekawa F
  • Journal Title: Frontiers in Neuroscience Volume: 10 Pages: 1-12
  • DOI: 10.3389/fnins.2016.00137
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Arsenic exposure induces unscheduled mitotic S phase entry coupled with cell death in mouse cortical astrocytes. (2016)
  • Author(s): Htike, NT., Maekawa, F., Soutome, H., Sano, K., Maejima, S., Aung, KH., Tokuda, M., Tsukahara, S.
  • Journal Title: Frontiers in Neuroscience Volume: 10 Pages: 297-297
  • DOI: 10.3389/fnins.2016.00297
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pubertal activation of estrogen receptor in the medial amygdala is essential for the full expression of male social behavior in mice (2016)
  • Author(s): Sano, K., Nakata, M., Musatov, S., Sakamoto, T., Morishita, M., Tsukahara, S., and Ogawa, S.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 113 Issue: 27 Pages: 7632-7637
  • DOI: 10.1073/pnas.1524907113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A comparative study of sex difference in calbindin neurons among mice, musk shrews, and Japanese quails. (2016)
  • Author(s): Moe, Y., Tanaka, T., Morishita, M., Ohata, R., Nakahara, C., Kawashima, T., Maekawa, F., Sakata, I., Sakai, T., Tsukahara, S.
  • Journal Title: Neuroscience Letters Volume: 631 Pages: 63-69
  • DOI: 10.1016/j.neulet.2016.08.018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Cloning of Ghrelin and Characteristics of Ghrelin-Producing Cells in the Gastrointestinal Tract of the Common Marmoset (Callithrix jacchus) (2016)
  • Author(s): Shota Takemi, Ichiro Sakata, Auvijit Saha Apu, Shinji Tsukahara, Satowa Yahashi, Goro Katsuura, Fumihiro Iwashige, Atsushi Akune, Akio Inui and Takafumi Sakai
  • Journal Title: Zoological Science Volume: 33(5) Issue: 5 Pages: 497-504
  • DOI: 10.2108/zs160020
  • NAID: 40020960657
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Sexually Dimorphic Area of the Dorsal Hypothalamus in Mice and Common Marmosets. (2016)
  • Author(s): Moe, Y., Kyi-Tha-Thu, C., Tanaka, T., Ito, H., Yahashi, S., Matsuda, K., Kawata, M., Katsuura, G., Iwashige, F., Sakata, I., Akune, A., Inui, A., Sakai, T., Ogawa, S., and Tsukahara, S.
  • Journal Title: Endocrinology Volume: 157 Issue: 12 Pages: 4817-4828
  • DOI: 10.1210/en.2016-1428
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sex differences in cells expressing green fluorescent protein under the control of the estrogen receptor  promoter in the hypothalamus of mice. (2015)
  • Author(s): Kyi Tha Thu C, Okoshi K, Ito H, Matsuda KI, Kawata M, Tsukahara S.
  • Journal Title: Neuroscience Research Volume: 101 Pages: 44-52
  • DOI: 10.1016/j.neures.2015.07.006
  • Peer Reviewed / Open Access
• [Journal Article] A neural connection between the central part of the medial preoptic nucleus and the bed nucleus of the stria terminalis to regulate sexual behavior in male rats. (2015)
  • Author(s): Maejima, S., Ohishi, N., Yamaguchi, S., Tsukahara, S.
  • Journal Title: Neuroscience Letters Volume: 606 Pages: 66-71
  • DOI: 10.1016/j.neulet.2015.08.047
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of Environmental Chemical Insult in Neuronal Cell Death and Cytoskeleton Damage (2015)
  • Author(s): Aung, KH., Tsukahara, S., Maekawa, F., Nohara, K., Nakamura, K., Tanoue, A.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 38 Issue: 8 Pages: 1109-1112
  • DOI: 10.1248/bpb.b14-00890
  • NAID: 130005090658
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access
• [Journal Article] Nano-sized secondary organic aerosol of diesel engine exhaust origin impairs olfactory-based spatial learning performance in preweaning mice. (2015)
  • Author(s): Win-Shwe, T-T., Kyi-Tha-Thu, C., Moe, Y., Maekawa, F., Yanagisawa, R., Furuyama, A., Tsukahara, S., Fujitani, Y., Hirano, S.
  • Journal Title: Nanomaterials Volume: 5 Pages: 1147-1162
  • Peer Reviewed
• [Presentation] マウスの背側視床下部-性的二型核（SDN-DH）の性分化における性ステロイドの働き (2017)
  • Author(s): 森下雅大、前嶋翔、塚原伸治
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松、静岡県浜松市
  • Year and Date: 2017-03-28
• [Presentation] 性行動を起こす雄ラットの視床下部矢状核における神経活性の変化 (2017)
  • Author(s): 内山慶、前嶋翔、山口祥平、松田賢一、塚原伸治
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松、静岡県浜松市
  • Year and Date: 2017-03-28
• [Presentation] 性経験は内側視索前野中心部のvgf nerve growth factor inducible（VGF）ニューロンの活性化を介して雄ラットの性的動機づけを亢進する (2017)
  • Author(s): 前嶋翔、内山慶、森下雅大、近藤保彦、塚原伸治
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松、静岡県浜松市
  • Year and Date: 2017-03-28
• [Presentation] 性的二型核マーカー Moxd1の内側視索前野における発現 (2017)
  • Author(s): 恒岡洋右、塚原伸治、吉田さちね、小田哲子、黒田優、船戸弘正
  • Organizer: 第122回日本解剖学会総会・学術集会
  • Place of Presentation: 長崎大学、長崎県長崎市
  • Year and Date: 2017-03-28
• [Presentation] 哺乳類における性的二型核の比較と性差形成機構 (2017)
  • Author(s): 塚原伸治
  • Organizer: 第46回ホミニゼーション研究会
  • Place of Presentation: 京都大学霊長類研究所、愛知県犬山市
  • Year and Date: 2017-03-24
  • Invited
• [Presentation] 脳の性差を構築する性ホルモンの作用機構 (2016)
  • Author(s): 塚原伸治
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] Sex and species differences in brain structure (2016)
  • Author(s): Tsukahara S.
  • Organizer: Mini-Symposium: Application of Recent Advances in Neuroscience to Environmental Health Research,
  • Place of Presentation: 国立環境研究所、茨城県つくば市
  • Year and Date: 2016-11-22
  • Invited
• [Presentation] Difference and homology of sexually dimorphic brain structures among quails, mice, musk shrews, and common marmosets (2016)
  • Author(s): Tsukahara S.
  • Organizer: Joint Events of 22nd International Congress of Zoology and 87th Meeting of Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-17
  • Int'l Joint Research / Invited
• [Presentation] Roles of testicular testosterone and ovarian estradiol during puberty on the formation of sexually dimorphic brain structures in mice (2016)
  • Author(s): Morishita M, Tsukahara S
  • Organizer: Joint Events of 22nd International Congress of Zoology and 87th Meeting of Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Involvement of neurons expressing corticotropin-releasing hormone (CRH) and vgf nerve growth factor inducible (VGF) in the central part of the medial preoptic nucleus on male sexual behavior in rats (2016)
  • Author(s): Maejima S, Yamaguchi S, Uchiyama K, Morishita M, Tsukahara S
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] マウス、スンクス、コモンマーモセットのカルビンディン発現細胞より構成される性的二型核 (2016)
  • Author(s): 田中相子、ヤダナモエ、森下雅大、矢橋里和、勝浦五郎、岩重記洋、阿久根淳、乾明夫、坂井貴文、塚原伸治
  • Organizer: 第43回日本神経内分泌学会学術集会
  • Place of Presentation: アクトシティ浜松、静岡県浜松市
  • Year and Date: 2016-10-14
• [Presentation] 性的二型核の性差構築に対する思春期の性ステロイドホルモンの影響 (2016)
  • Author(s): 森下雅大、塚原伸治
  • Organizer: 第43回日本神経内分泌学会学術集会
  • Place of Presentation: アクトシティ浜松、静岡県浜松市
  • Year and Date: 2016-10-14
• [Presentation] Gonadal steroid action on sex-specific formation of the brain (2016)
  • Author(s): Tsukahara S.
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society（第39回日本神経科学大会）
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2016-07-21
  • Invited
• [Presentation] A comparative study of morphological sex differences in the brain of mice, suncus, and common marmosets (2016)
  • Author(s): Tanaka T, Yadanar M, Morishita M, Nakahara C, Yahashi S, Sakata I, Katsuura G, Iwashige F, Akune A, Inui A, Sakai T, Tsukahara S
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society（第39回日本神経科学大会）
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2016-07-20
• [Presentation] Involvement of neurons expressing corticotropin-releasing hormone (CRH) and vgf nerve growth factor inducible (VGF) in the central part of the medial preoptic nucleus on sexual behavior in male rats (2016)
  • Author(s): Maejima S, Yamaguchi S, Uchiyama K, Morishita M, Tsukahara S
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society（第39回日本神経科学大会）
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2016-07-20
• [Presentation] Arsenic exposure induces forced S phase entry linked to cell death in mouse cortical astrocytes (2016)
  • Author(s): Nang Thinn Thinn Htike, Maekawa F, Soutome H, Sano K, Maejima S, Kyaw Htet Aung, Tsukahara S
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society（第39回日本神経科学大会）
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2016-07-20
• [Presentation] Lactational exposure to benzo[a]pyrene (BaP) on neuroimmune biomarkers in hippocampus of an allergic asthmatic mouse model (2016)
  • Author(s): Win-Shwe Tin-Tin, Nan Thin Thin Htike, Tsukahara S, Koike E, Yanagisawa R
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society（第39回日本神経科学大会）
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2016-07-20
• [Presentation] Region-specific actions of sex steroids on the formation of morphological sex difference in the brain. (2016)
  • Author(s): Tsukahara, S., Kanaya, M.
  • Organizer: 93rd Annual Meeting of the Physiological Society of Japan（第93回日本生理学会大会）
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2016-03-22
  • Invited
• [Presentation] Involvement of a novel female-biased sexually dimorphic area of the hypothalamus on maternal behavior in mice. (2015)
  • Author(s): Moe, Y., Kyi-Tha-Thu, C., Ito, H., Tsukahara, S.
  • Organizer: 第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会合同大会
  • Place of Presentation: 広島アステールプラザ、広島
  • Year and Date: 2015-12-11
• [Presentation] Effects of parental care and mother-pup separation on maternal behavior in primiparous female rats. (2015)
  • Author(s): Hoshikawa, Y., Kamada, A., Morita, S., Tsukahara, S.
  • Organizer: 第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会合同大会
  • Place of Presentation: 広島アステールプラザ、広島
  • Year and Date: 2015-12-11
• [Presentation] Effects of maternal experience on dendritic spine and glutamate receptor expression in postpartum rats. (2015)
  • Author(s): Morita, S., Kamada, A., Maejima, S., Tsukahara, S.
  • Organizer: 第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会合同大会
  • Place of Presentation: 広島アステールプラザ、広島
  • Year and Date: 2015-12-11
• [Presentation] Involvement of vgf nerve growth factor inducible (VGF) in the medial preoptic nucleus (MPN) on sexual behavior in male rats. (2015)
  • Author(s): Maejima, S., Yamaguchi, S., Morishita, M., Tsukahara, S.
  • Organizer: 第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会合同大会
  • Place of Presentation: 広島アステールプラザ、広島
  • Year and Date: 2015-12-11
• [Presentation] Sexually dimorphic nuclei (SDNs) in the brain of suncus: A comparative study between mice and suncus. (2015)
  • Author(s): Tanaka, T., Moe, Y., Morishita, M., Maejima, S., Nakahara, C., Sakata, I., Sakai, T., Tsukahara, S.
  • Organizer: 第40回日本比較内分泌学会大会・日本比較生理生化学会第37回大会合同大会
  • Place of Presentation: 広島アステールプラザ、広島
  • Year and Date: 2015-12-11
• [Presentation] A sex difference in green fluorescent protein expression under the control of the estrogen receptor-alpha promoter in the hypothalamus of mice. (2015)
  • Author(s): Kyi-Tha-Thu, C., Okoshi, K., Ito, H., Matsuda, K-I., Kawata, M., Tsukahara, S.
  • Organizer: 8th Congress Federation of the Asian and Oceanian Physiological Societies
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Male-biased sexually dimorphic nuclei found in the brain of Suncus murinicus. (2015)
  • Author(s): Moe, Y., Tanaka, T., Morishita, M., Nakahara, C., Sakata, I., Sakai, T., Tsukahara, S.
  • Organizer: 8th Congress Federation of the Asian and Oceanian Physiological Societies
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] 脳の性差：新たに見つかった視床下部の性的二型領域 (2015)
  • Author(s): 塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
  • Invited
• [Presentation] 分界条床核へ投射する内側視索前核中心部ニューロンの雄性行動制御における関与 (2015)
  • Author(s): 前嶋翔、大石直哉、山口祥平、塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
• [Presentation] 性的二型核の性差形成における周生期と思春期の性ステロイド作用 (2015)
  • Author(s): 金谷萌子、津田夢芽子、佐越祥子、永田和代、森本千尋、Chaw Kyi Tha Thu、戸田勝巳、加藤茂明、小川園子、塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
• [Presentation] カルビンディン発現ニューロンにより構成される性的二型核の比較解剖学的解析 (2015)
  • Author(s): 田中相子、中原千尋、Yadanar Moe、前嶋翔、坂田一郎、坂井貴文、塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
• [Presentation] 初産雌ラットの産後初期における仔育て経験の母性行動促進効果 (2015)
  • Author(s): 星川祐美、鎌田亜里紗、森田沙也佳、塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
• [Presentation] 内側視索前核ニューロンのスパインと神経突起に対する仔育ての影響 (2015)
  • Author(s): 鎌田亜里紗、森田沙也佳、前嶋翔、沖野恵理、塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
• [Presentation] 初産雌ラットの内側視索前核ニューロンの形態およびグルタミン酸受容体に対する母仔接触と仔育ての影響 (2015)
  • Author(s): 森田沙也佳、鎌田亜里紗、前嶋翔、塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
• [Presentation] 雄ラットにおける内側視索前核中心部の神経活性に対する性経験の影響 (2015)
  • Author(s): 山口祥平、前嶋翔、内山慶、阿部雄太、塚原伸治
  • Organizer: 第42回日本神経内分泌学・第23回日本行動神経内分泌研究会合同学術集会
  • Place of Presentation: 戦災復興記念館、仙台
  • Year and Date: 2015-09-18
• [Presentation] Effects of arsenic exposure on cell proliferation and viablity of astrocytes. (2015)
  • Author(s): Thin-Thin-Htike, N., Soutome, H., Sano, K., Aung, KH., Maekawa, F., Nohara, K. Tsukahara, S.
  • Organizer: 51st Congress of the European Societies of Toxicology
  • Place of Presentation: Porto, Portugal
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Book] みんなの生命科学 (2016)
  • Author(s): 北口哲也、塚原伸治、坪井貴司、前川文彦
  • Total Pages: 213
  • Publisher: 化学同人
• [Book] ホルモンから見た生命現象と進化シリーズIV：求愛・性行動と脳の性分化（小林牧人、小澤一史、棟方有宗、編）、第5章 齧歯類の性行動と脳の性分化 (2016)
  • Author(s): 浜崎浩子、小林牧人、棟方有宗、小澤一史、豊田ふみよ、塚原伸治、吉国道庸
  • Total Pages: 130
  • Publisher: 裳華房