A pseudopod-like nano structures are involved in wound healing: observations by NanoSuit method in field-emission scanning electron microscopy.
| Project/Area Number |
15K14558
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HIRAKAWA Satoshi 浜松医科大学, 医学部・皮膚科学講座, 准教授 (50419511)
KAWASAKI Hideya 浜松医科大学, 医学部・再生・感染病理学講座, 助教 (90397381)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 電界放出形走査電子顕微鏡 / 高真空 / 含水状態 / NanoSuit / 電子顕微鏡 / 生きたまま |
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| Outline of Final Research Achievements |
We have previously described a method that allows the investigator to keep a variety of specimens wet in the high vacuum environment of the electron microscope by encasing the organisms in a thin, vacuum-proof suit, the ‘NanoSuit’. In the present study, to examine the function of pseudopod-like nano structures in wound healing, which had been detected by NanoSuit method, we analyzed the regenerative tissues in hydra. The results provide evidences that the number of nano structures changed with time in the beginning of the tissue repair, which process seemed consistent with wound closing. Histological and pharmacological studies suggest that actin filaments play an important role in the regulation of the nano structures. Furthermore, we found that such structures were detected in other animals in regenerative or growing processes. Taken together, the results suggest the possibility that these nano structures would contribute to animal development and/or regeneration.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] A modified ‘NanoSuit’ preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy.2017
Author(s)
Takaku Y, Suzuki H, Kawasaki H, Ohta I, Ishii D, Hirakawa S, Tsutsui T, Matsumoto H, Takehara S, Nakane C, Sakaida K, Suzuki C, Muranaka Y, Kikuchi H, Konno H, Shimomura M, Hariyama T
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Journal Title
R Soc Open Sci
Volume: なし
Issue: 3
Pages: 160887-160887
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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