| Project/Area Number |
15K14559
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Nagoya University |
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Principal Investigator |
SASAKI NARIE 名古屋大学, 理学研究科, 准教授 (20359699)
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| Co-Investigator(Renkei-kenkyūsha) |
MORIYAMA Yosuke (ITOU Hideto) 藤田保健衛生大学, 医学部, 助教 (00452532)
ITAMI Kenichirou 名古屋大学, トランスフォーマティブ生命分子研究所, 教授 (80311728)
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| Research Collaborator |
URAKAWA Naoki
NAKAMURA Satoru
SASAKI Taeko
TANAKA Yoriko
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 母性遺伝 / ミトコンドリア / ヌクレアーゼ / DNAヌクレアーゼ |
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| Outline of Final Research Achievements |
In many eukaryotes, mitochondrial DNA (mtDNA) is inherited maternally, which is called maternal inheritance. For maternal inheritance, selective digestion of mtDNA in paternal mitochondria is essential. However, it is unknown how mtDNA in paternal mitochondria is selectively digested by DNase after zygosis or fertilization. In this study, to identify DNase involved in maternal inheritance of mtDNA, we tried to fractionate paternal mitochondria which had no mtDNA from zygote of Physarum polycephalu using cell sorter. Since mitochondria were destroyed in the cell sorter, we couldn’t fractionate. Then, we performed proteome analysis of isolated mitodhondria from zygote by nanoLC-MS/MS. We identified nine DNases in isolated mitochondria from zygotes that had mitochondria without mtDNA.
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